DDX21 antibody (C-Term)

Catalog No. ABIN2775198

Product Details anti-DDX21 Antibody

Target: DDX21 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 21 (DDX21))

Binding Specificity: C-Term

Reactivity: Human, Rat, Horse, Dog, Pig, Guinea Pig, Cow, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDX21 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: FRGQRDGNRR FRGQREGSRG PRGQRSGGGN KSNRSQNKGQ KRSFSKAFGQ

Predicted Reactivity: Cow: 86%, Dog: 93%, Guinea Pig: 86%, Horse: 93%, Human: 100%, Pig: 93%, Rabbit: 93%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against DDX21. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human DDX21

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 783 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-DDX21 antibody (ABIN2775198)

Miyazawa, Yoshikawa, Magae, Ishikawa, Izumikawa, Terukina, Suzuki, Nakamura-Fujiyama, Miura, Hayano, Komatsu, Isobe, Takahashi: "Human cell growth regulator Ly-1 antibody reactive homologue accelerates processing of preribosomal RNA." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 19, Issue 4, pp. 273-86, (2014) (PubMed).

Romanova, Grand, Zhang, Rayner, Katoku-Kikyo, Kellner, Kikyo: "Critical role of nucleostemin in pre-rRNA processing." in: The Journal of biological chemistry, Vol. 284, Issue 8, pp. 4968-77, (2009) (PubMed).

Target Details for DDX21

Target: DDX21 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 21 (DDX21))

Alternative Name: DDX21 (DDX21 Products)

Synonyms: MGC130693 antibody, DDX21 antibody, cb960 antibody, wu:fi30c07 antibody, wu:fi34d08 antibody, GUA antibody, GURDB antibody, RH-II/GU antibody, RH-II/GuA antibody, Ddx21a antibody, AI255159 antibody, AL022742 antibody, D10Ertd645e antibody, D10Wsu42e antibody, DEAD-box helicase 21 L homeolog antibody, DExD-box helicase 21 antibody, DEAD-box helicase 21 antibody, DEAD (Asp-Glu-Ala-Asp) box helicase 21 antibody, DEAD (Asp-Glu-Ala-Asp) box polypeptide 21 antibody, ddx21.L antibody, DDX21 antibody, ddx21 antibody, Ddx21 antibody

Background: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX21 encodes a DEAD box protein, which is an antigen recognized by autoimmune antibodies from a patient with watermelon stomach disease. This protein unwinds double-stranded RNA, folds single-stranded RNA, and may play important roles in ribosomal RNA biogenesis, RNA editing, RNA transport, and general transcription.
Alias Symbols: GUA, GURDB, RH-II/GU, RH-II/GuA
Protein Interaction Partner: HUWE1, PA2G4, KPNA3, CEP250, SUMO2, SUMO3, STAU1, IVNS1ABP, UBC, MDM2, LGR4, SUMO1, ERG, WWOX, EED, rev, SRPK2, SRPK3, FBXO6, TARDBP, LYN, AICDA, PAN2, vif, WHSC1, VCAM1, ITGA4, IL7R, FN1, CSNK2A1, YWHAE, SMURF1, H2AFX, APTX, ESR1, FTSJ3, PCDHA2, RRP7A, R
Protein Size: 783

Molecular Weight: 86 kDa

Gene ID: 9188

NCBI Accession: NM_004728, NP_004719

UniProt: Q9NR30

Pathways: SARS-CoV-2 Protein Interactome