RENT1/UPF1 antibody (Middle Region)

Catalog No. ABIN2775184

Product Details anti-RENT1/UPF1 Antibody

Target: RENT1/UPF1 (UPF1) (UPF1 Regulator of Nonsense Transcripts Homolog (UPF1))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Zebrafish (Danio rerio), Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RENT1/UPF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: RGTPKGKTGR GGRQKNRFGL PGPSQTNLPN SQASQDVASQ PFSQGALTQG

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 79%, Horse: 93%, Human: 100%, Mouse: 93%, Rat: 93%, Zebrafish: 83%

Characteristics: This is a rabbit polyclonal antibody against UPF1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human UPF1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1118 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for RENT1/UPF1

Target: RENT1/UPF1 (UPF1) (UPF1 Regulator of Nonsense Transcripts Homolog (UPF1))

Alternative Name: UPF1 (UPF1 Products)

Synonyms: HUPF1 antibody, NORF1 antibody, RENT1 antibody, pNORF1 antibody, smg-2 antibody, B430202H16Rik antibody, PNORF-1 antibody, Rent1 antibody, Upflp antibody, rent1 antibody, wu:fi40f07 antibody, wu:fj48a01 antibody, zgc:55472 antibody, Tb05.3C6.50 antibody, AO090012000584 antibody, hupf1 antibody, norf1 antibody, pnorf1 antibody, upf1 antibody, UPF1, RNA helicase and ATPase antibody, UPF1 regulator of nonsense transcripts homolog (yeast) antibody, upf1 regulator of nonsense transcripts homolog (yeast) antibody, regulator of nonsense transcripts 1 antibody, Regulator of nonsense transcripts 1 antibody, UPF1 regulator of nonsense transcripts homolog S homeolog antibody, UPF1 antibody, Upf1 antibody, upf1 antibody, Tc00.1047053511317.30 antibody, Tb927.5.2140 antibody, TVAG_453890 antibody, TVAG_237840 antibody, AOR_1_1018194 antibody, HPB8_739 antibody, smg-2 antibody, upf1.S antibody

Background: UPF1 is a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. UPF1 is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene.This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: FLJ43809, FLJ46894, HUPF1, KIAA0221, NORF1, RENT1, pNORF1, smg-2
Protein Interaction Partner: UBC, SUMO2, STAU1, CDC37, IVNS1ABP, MOV10, DNA2, POP1, LIN28A, UPF3B, STAU2, UPF2, EZH2, BMI1, SUZ12, RNF2, GNPTG, RHOXF2, ACSS2, NADSYN1, PLEKHB2, PLEKHA5, TARDBP, RPRD2, ABHD16A, NDUFB10, DXO, SMG1, RUVBL2, RBM8A, SMG7, EIF4A3, RUVBL1, GSPT2, SMG5, GSPT
Protein Size: 1118

Molecular Weight: 123 kDa

Gene ID: 5976

NCBI Accession: NM_002911, NP_002902

UniProt: Q92900

Pathways: SARS-CoV-2 Protein Interactome