MCM4 antibody (N-Term)
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- Target See all MCM4 Antibodies
- MCM4 (Minichromosome Maintenance Deficient 4 (MCM4))
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Binding Specificity
- N-Term
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Reactivity
- Human, Mouse, Rat, Rabbit, Cow, Guinea Pig, Dog, Horse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This MCM4 antibody is un-conjugated
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Application
- Western Blotting (WB)
- Sequence
- SRVEGTPRSG VRGTPVRQRP DLGSAQKGLQ VDLQSDGAAA EDIVASEQSL
- Predicted Reactivity
- Cow: 86%, Dog: 92%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 93%, Rabbit: 93%, Rat: 93%
- Characteristics
- This is a rabbit polyclonal antibody against MCM4. It was validated on Western Blot using a cell lysate as a positive control.
- Purification
- Protein A purified
- Immunogen
- The immunogen is a synthetic peptide directed towards the N terminal region of human MCM4
- Top Product
- Discover our top product MCM4 Primary Antibody
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- Application Notes
- Optimal working dilutions should be determined experimentally by the investigator.
- Comment
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Antigen size: 863 AA
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
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- Target
- MCM4 (Minichromosome Maintenance Deficient 4 (MCM4))
- Alternative Name
- MCM4 (MCM4 Products)
- Synonyms
- cdc21 antibody, CDC21 antibody, CDC54 antibody, NKCD antibody, NKGCD antibody, P1-CDC21 antibody, hCdc21 antibody, 19G antibody, AI325074 antibody, AU045576 antibody, Cdc21 antibody, Mcmd4 antibody, mKIAA4003 antibody, mcdc21 antibody, cb1025 antibody, fc12c09 antibody, fj85g09 antibody, hm:zeh1616 antibody, wu:fc12c09 antibody, wu:fj85g09 antibody, zeh1616 antibody, minichromosome maintenance complex component 4 L homeolog antibody, minichromosome maintenance complex component 4 S homeolog antibody, minichromosome maintenance complex component 4 antibody, mcm4.L antibody, mcm4.S antibody, MCM4 antibody, mcm4 antibody, Mcm4 antibody
- Background
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The protein encoded by MCM4 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported.
Alias Symbols: CDC21, CDC54, hCdc21, P1-CDC21
Protein Interaction Partner: HUWE1, UBC, PCNA, SUMO2, FKBP5, MCMBP, MDM2, POLK, CLUH, NCKAP1, CAP1, CAP2, EIF3B, MCM2, KPNA3, PRMT1, FKBP4, MCM7, MCM6, FBXW4, FBXO46, ITGA4, CDC5L, gag-pol, ORC6, CCNA1, VCAM1, DBF4, CDC7, LIG4, MCM5, MCM3, DCTN2, SF3A1, UBD, NEDD8, TONSL, SIRT7, ASF1
Protein Size: 863 - Molecular Weight
- 95 kDa
- Gene ID
- 4173
- NCBI Accession
- NM_182746, NP_877423
- UniProt
- P33991
- Pathways
- DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA
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