GJB2 antibody (Middle Region)

Catalog No. ABIN2774858

Product Details anti-GJB2 Antibody

Target: GJB2 (Gap Junction Protein, beta 2, 26kDa (GJB2))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Dog, Horse, Cow, Guinea Pig, Rabbit, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GJB2 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: STPALLVAMH VAYRRHEKKR KFIKGEIKSE FKDIEEIKTQ KVRIEGSLWW

Predicted Reactivity: Cow: 86%, Dog: 100%, Guinea Pig: 86%, Horse: 100%, Human: 100%, Mouse: 86%, Rabbit: 79%, Rat: 93%, Sheep: 86%

Characteristics: This is a rabbit polyclonal antibody against GJB2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human GJB2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 226 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for GJB2

Target: GJB2 (Gap Junction Protein, beta 2, 26kDa (GJB2))

Alternative Name: GJB2 (GJB2 Products)

Synonyms: ppk antibody, cx26 antibody, dfna3 antibody, dfnb1 antibody, nsrd1 antibody, dfna3a antibody, dfnb1a antibody, MGC53062 antibody, connexin-26 antibody, GJB2 antibody, AI325222 antibody, Cnx26 antibody, Cx26 antibody, Gjb-2 antibody, CXN-26 antibody, CX26 antibody, DFNA3 antibody, DFNA3A antibody, DFNB1 antibody, DFNB1A antibody, HID antibody, KID antibody, NSRD1 antibody, PPK antibody, gap junction protein beta 2 L homeolog antibody, gap junction protein beta 2 antibody, gap junction protein, beta 2 antibody, gjb2.L antibody, GJB2 antibody, Gjb2 antibody

Background: Gap junctions were first characterized by electron microscopy as regionally specialized structures on plasma membranes of contacting adherent cells. These structures were shown to consist of cell-to-cell channels. Proteins, called connexins, purified from fractions of enriched gap junctions from different tissues differ. The connexins are designated by their molecular mass. Another system of nomenclature divides gap junction proteins into 2 categories, alpha and beta, according to sequence similarities at the nucleotide and amino acid levels. For example, CX43 (MIM 121014) is designated alpha-1 gap junction protein, whereas CX32 (GJB1, MIM 304040) and CX26 are called beta-1 and beta-2 gap junction proteins, respectively. This nomenclature emphasizes that CX32 and CX26 are more homologous to each other than either of them is to CX43.
Alias Symbols: HID, KID, PPK, CX26, DFNA3, DFNB1, NSRD1, DFNA3A, DFNB1A
Protein Interaction Partner: CD14, CNST, FBXO2, SKP1, UBC, GJB6, CAV1, GJB1,
Protein Size: 226

Molecular Weight: 25 kDa

Gene ID: 2706

NCBI Accession: NM_004004, NP_003995

UniProt: P29033

Pathways: Sensory Perception of Sound, Cell-Cell Junction Organization