NUCB2 antibody (Middle Region)

Catalog No. ABIN2774832

Product Details anti-NUCB2 Antibody

Target: NUCB2 (Nucleobindin 2 (NUCB2))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Dog, Horse, Pig, Rabbit, Cow, Guinea Pig, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NUCB2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: MMKEHERREY LKTLNEEKRK EEESKFEEMK KKHENHPKVN HPGSKDQLKE

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 86%, Horse: 100%, Human: 100%, Mouse: 93%, Pig: 100%, Rabbit: 93%, Rat: 93%, Yeast: 91%

Characteristics: This is a rabbit polyclonal antibody against NUCB2. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NUCB2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 420 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-NUCB2 antibody (ABIN2774832)

Takagi, Miki, Tanaka, Hashimoto, Watanabe, Sasano, Ito, Suzuki: "Nucleobindin 2 (NUCB2) in human endometrial carcinoma: a potent prognostic factor associated with cell proliferation and migration." in: Endocrine journal, Vol. 63, Issue 3, pp. 287-99, (2016) (PubMed).

Suzuki, Takagi, Miki, Onodera, Akahira, Ebata, Ishida, Watanabe, Sasano, Suzuki: "Nucleobindin 2 in human breast carcinoma as a potent prognostic factor." in: Cancer science, Vol. 103, Issue 1, pp. 136-43, (2012) (PubMed).

Target Details for NUCB2

Target: NUCB2 (Nucleobindin 2 (NUCB2))

Alternative Name: NUCB2 (NUCB2 Products)

Synonyms: nefa antibody, MGC97715 antibody, NUCB2 antibody, nucleobindin-2 antibody, NEFA antibody, AI607786 antibody, Calnuc antibody, Nefa antibody, Nesfatin-1 antibody, p54 antibody, nucleobindin 2 antibody, nucleobindin 2 L homeolog antibody, nucb2 antibody, NUCB2 antibody, Nucb2 antibody, nucb2.L antibody

Background: Nucleobindin-2 is a calcium-binding EF-hand protein.
Alias Symbols: NEFA
Protein Interaction Partner: UBC, NEDD8, TUBB6, CAMK1D, NAE1, HSPA1L, CLU, GNAS, GNAI3, GADD45A, XPO1, SMARCD1, CUL2, NR1I2, NDN, STAR,
Protein Size: 420

Molecular Weight: 46 kDa

Gene ID: 4925

NCBI Accession: NM_005013, NP_005004

UniProt: P80303