ATF3 antibody (Middle Region)

Catalog No. ABIN2774825

Product Details anti-ATF3 Antibody

Target: ATF3 (Activating Transcription Factor 3 (ATF3))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio), Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATF3 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: PLGVSITKAE VAPEEDERKK RRRERNKIAA AKCRNKKKEK TECLQKESEK

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 86%, Rabbit: 93%, Rat: 93%, Yeast: 75%, Zebrafish: 80%

Characteristics: This is a rabbit polyclonal antibody against ATF3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ATF3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 181 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for ATF3

Target: ATF3 (Activating Transcription Factor 3 (ATF3))

Alternative Name: ATF3 (ATF3 Products)

Synonyms: zgc:55526 antibody, ATF3 antibody, LRG-21 antibody, LRF-1 antibody, LRFI antibody, atf-3 antibody, activating transcription factor 3 antibody, activating transcription factor 3 L homeolog antibody, atf3 antibody, ATF3 antibody, Atf3 antibody, atf3.L antibody

Background: This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. ATF3 represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters.Activating transcription factor 3 is a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. Multiple transcript variants encoding two different isoforms have been found for this gene. The longer isoform represses rather than activates transcription from promoters with ATF binding elements. The shorter isoform (deltaZip2) lacks the leucine zipper protein-dimerization motif and does not bind to DNA, and it stimulates transcription presumably by sequestering inhibitory co-factors away from the promoter. It is possible that alternative splicing of the ATF3 gene may be physiologically important in the regulation of target genes.
Alias Symbols: -
Protein Interaction Partner: SUV39H1, BATF3, MAFF, CREB3, FOSL1, MAFG, JUNB, JUN, FOS, DDIT3, DBP, ATF2, CEBPG, CEBPE, CEBPA, ATF4, ATF3, BATF, HSP90AA1, SRA1, ZNF212, ID3, FHL2, SUMO1, SUMO3, HDAC10, HDAC5, HDAC6, HDAC4, HDAC3, HDAC1, SMARCA4, PDX1, ACTB, UBC, SUMO2, STAT1, MDM2, SM
Protein Size: 181

Molecular Weight: 20 kDa

Gene ID: 467

NCBI Accession: NM_001674, NP_001665

UniProt: P18847

Pathways: Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Unfolded Protein Response