RNF128 antibody (C-Term)

Catalog No. ABIN2774780

Product Details anti-RNF128 Antibody

Target: RNF128 (Ring Finger Protein 128 (RNF128))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Horse, Rabbit, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF128 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: LEEHVQSTNE SLQLVNHEAN SVAVDVIPHV DNPTFEEDET PNQETAVREI

Predicted Reactivity: Cow: 86%, Dog: 93%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 93%, Rabbit: 93%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against RNF128. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human RNF128

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 428 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for RNF128

Target: RNF128 (Ring Finger Protein 128 (RNF128))

Alternative Name: RNF128 (RNF128 Products)

Background: RNF128 is a type I transmembrane protein that localizes to the endocytic pathway. This protein contains a RING zinc-finger motif and has been shown to possess E3 ubiquitin ligase activity. Expression of RNF128 in retrovirally transduced T cell hybridoma significantly inhibits activation-induced IL2 and IL4 cytokine production. Induced expression of RNF128 was observed in anergic CD4(+) T cells, which suggested a role in the induction of anergic phenotype.The protein encoded by this gene is a type I transmembrane protein that localizes to the endocytic pathway. This protein contains a RING zinc-finger motif and has been shown to possess E3 ubiquitin ligase activity. Expression of this gene in retrovirally transduced T cell hybridoma significantly inhibits activation-induced IL2 and IL4 cytokine production. Induced expression of this gene was observed in anergic CD4(+) T cells, which suggested a role in the induction of anergic phenotype. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
Alias Symbols: FLJ23516, GRAIL
Protein Interaction Partner: CEP76, APPBP2, TP53, UBE2H, UBE2E1, UBE2D1, Arhgdia, Arhgdib, RNF128, UBE2T, UBE2I, UBE2N, CD40LG, OTUB1, USP8,
Protein Size: 428

Molecular Weight: 47 kDa

Gene ID: 79589

NCBI Accession: NM_194463, NP_919445

UniProt: Q8TEB7