MARCH5 antibody (N-Term)

Catalog No. ABIN2774746

Product Details anti-MARCH5 Antibody

Target: MARCH5 (Membrane-Associated Ring Finger (C3HC4) 5 (MARCH5))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Horse, Rabbit, Dog, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MARCH5 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: CRGSTKWVHQ ACLQRWVDEK QRGNSTARVA CPQCNAEYLI VFPKLGPVVY

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against MARCH5. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MARCH5

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 278 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-MARCH5 antibody (ABIN2774746)

Cilenti, Ambivero, Ward, Alnemri, Germain, Zervos: "Inactivation of Omi/HtrA2 protease leads to the deregulation of mitochondrial Mulan E3 ubiquitin ligase and increased mitophagy." in: Biochimica et biophysica acta, Vol. 1843, Issue 7, pp. 1295-307, (2014) (PubMed).

Target Details for MARCH5

Target: MARCH5 (Membrane-Associated Ring Finger (C3HC4) 5 (MARCH5))

Alternative Name: MARCH5 (MARCH5 Products)

Synonyms: march5 antibody, MARCH5 antibody, mitol antibody, rnf153 antibody, march-v antibody, zgc:162966 antibody, MARCH-V antibody, Mar-05 antibody, MITOL antibody, RNF153 antibody, 1810015H18Rik antibody, 2310008I22Rik antibody, 2700055A20Rik antibody, 5730499H23Rik antibody, E130202O05Rik antibody, Marchv antibody, Rnf153 antibody, membrane-associated ring finger (C3HC4) 5 antibody, membrane associated ring-CH-type finger 5 antibody, membrane associated ring-CH-type finger 5 L homeolog antibody, march5 antibody, MARCH5 antibody, march5.L antibody, March5 antibody

Background: MARCH5 is an ubiquitin ligase of the mitochondrial outer membrane that plays a role in the control of mitochondrial morphology by regulating mitofusin-2 (MFN2) and DRP1 (DNM1L). MARCH5 is a ubiquitin ligase of the mitochondrial outer membrane that plays a role in the control of mitochondrial morphology by regulating mitofusin-2 (MFN2, MIM 608507) and DRP1 (DNM1L, MIM 603850) (Nakamura et al., 2006 [PubMed 16936636]).[supplied by OMIM]. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-1502 AK000452.1 3-1504 1503-1863 BC015480.1 1463-1823 1864-1901 BE393612.1 438-475 1902-3713 BC015480.1 1852-3663 3714-3941 BC015480.1 3669-3896
Alias Symbols: FLJ20445, MARCH-V, MITOL, RNF153
Protein Interaction Partner: FATE1, UBE2W, UBC, DNM1L, MFN2, UBE2E1, FIS1, UBE2E3, MFN1, PARK2, MARCH5, CCNB1, UBE2D3, UBE2D2, UBE2D1, MAP1B, KIAA0368, SOD1, MAVS, TANK, TRAF6, AKTIP, UBE2T, UBE2N, UBE2I, UTRN,
Protein Size: 278

Molecular Weight: 31 kDa

Gene ID: 54708

NCBI Accession: NM_017824, NP_060294

UniProt: Q9NX47