RNF43 antibody (Middle Region)

Catalog No. ABIN2774743

Product Details anti-RNF43 Antibody

Target: RNF43 (Ring Finger Protein 43 (RNF43))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF43 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: DFDPLVYCSP KGDPQRVDMQ PSVTSRPRSL DSVVPTGETQ VSSHVHYHRH

Predicted Reactivity: Cow: 86%, Dog: 93%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against RNF43. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RNF43

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 783 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-RNF43 antibody (ABIN2774743)

Sugiura, Yamaguchi, Miyamoto: "A cancer-associated RING finger protein, RNF43, is a ubiquitin ligase that interacts with a nuclear protein, HAP95." in: Experimental cell research, Vol. 314, Issue 7, pp. 1519-28, (2008) (PubMed).

Target Details for RNF43

Target: RNF43 (Ring Finger Protein 43 (RNF43))

Alternative Name: RNF43 (RNF43 Products)

Background: RNF43 is a HAP95 (AKAP8L) binding ubiquitin ligase that promotes cell growth and is upregulated in colon cancer.
Alias Symbols: DKFZp781H02126, DKFZp781H0392, FLJ20315, MGC125630, RNF124, URCC
Protein Interaction Partner: RSPO1, SFPQ, NONO, HECW1, TP53, UBE2D4, KDM5B, UBE2E3, UBE2N, UBE2E1, UBE2D3, UBE2D2, UBE2D1, AKAP8L, UBC,
Protein Size: 783

Molecular Weight: 86 kDa

Gene ID: 54894

NCBI Accession: NM_017763, NP_060233

UniProt: Q68DV7

Pathways: WNT Signaling