FBXO5 antibody (C-Term)

Catalog No. ABIN2774700

Product Details anti-FBXO5 Antibody

Target: FBXO5 (F-Box Protein 5 (FBXO5))

Binding Specificity: C-Term

Reactivity: Human, Dog, Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FBXO5 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: ASVQKSAAQT SLKKDAQTKL SNQGDQKGST YSRHNEFSEV AKTLKKNESL

Predicted Reactivity: Dog: 85%, Human: 100%, Pig: 79%, Rabbit: 92%

Characteristics: This is a rabbit polyclonal antibody against FBXO5. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human FBXO5

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 447 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-FBXO5 antibody (ABIN2774700)

Estrabaud, Le Rouzic, Lopez-Vergès, Morel, Belaïdouni, Benarous, Transy, Berlioz-Torrent, Margottin-Goguet: "Regulated degradation of the HIV-1 Vpu protein through a betaTrCP-independent pathway limits the release of viral particles." in: PLoS pathogens, Vol. 3, Issue 7, pp. e104, (2007) (PubMed).

Target Details for FBXO5

Target: FBXO5 (F-Box Protein 5 (FBXO5))

Alternative Name: FBXO5 (FBXO5 Products)

Synonyms: EMI1 antibody, FBX5 antibody, Fbxo31 antibody, 2510044I10Rik antibody, C85305 antibody, Emi1 antibody, emi1 antibody, fc65h02 antibody, wu:fc65h02 antibody, wu:fe06e07 antibody, wu:fz79f03 antibody, zgc:136397 antibody, zgc:158541 antibody, fbx5 antibody, Fbxo5 antibody, ACYPI007854 antibody, fbxo5 antibody, F-box protein 5 antibody, F-box only protein 5 antibody, F-box protein 5 L homeolog antibody, F-box protein 5 S homeolog antibody, FBXO5 antibody, Fbxo5 antibody, fbxo5 antibody, fbxo5.L antibody, fbxo5.S antibody

Background: FBXO5 is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein belongs to the Fbxs class and is similar to xenopus early mitotic inhibitor-1 (Emi1), which is a mitotic regulator that interacts with Cdc20 and inhibits the anaphase promoting complex. Alternatively spliced transcript variants encoding different isoforms have been identified.This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. This protein is similar to xenopus early mitotic inhibitor-1 (Emi1), which is a mitotic regulator that interacts with Cdc20 and inhibits the anaphase promoting complex. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: EMI1, FBX5, Fbxo31
Protein Interaction Partner: FBXW11, UBE2S, SKP1, UBC, TPM3, NEDD8, BRCA1, BARD1, ANAPC1, ANAPC11, ANAPC7, ANAPC5, FZR1, ANAPC4, ANAPC2, CDC16, CDC23, CDC27, CDC20, CDK1, MYC, Gm9174, BTRC, PLK1, EVI5,
Protein Size: 447

Molecular Weight: 50 kDa

Gene ID: 26271

NCBI Accession: NM_012177, NP_036309

UniProt: Q9UKT4

Pathways: Mitotic G1-G1/S Phases