FBXO4 antibody (Middle Region)

Catalog No. ABIN2774698

Product Details anti-FBXO4 Antibody

Target: FBXO4 (F-Box Protein 4 (FBXO4))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Guinea Pig, Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FBXO4 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: TSAVNKMFSR HNEGDDQQGS RYSVIPQIQK VCEVVDGFIY VANAEAHKSK

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 92%, Horse: 100%, Human: 100%, Mouse: 92%, Pig: 100%, Rabbit: 100%, Rat: 92%

Characteristics: This is a rabbit polyclonal antibody against FBXO4. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human FBXO4

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 307 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-FBXO4 antibody (ABIN2774698)

Ewing, Chu, Elisma, Li, Taylor, Climie, McBroom-Cerajewski, Robinson, OConnor, Li, Taylor, Dharsee, Ho, Heilbut, Moore, Zhang, Ornatsky, Bukhman, Ethier, Sheng, Vasilescu, Abu-Farha, Lambert, Duewel et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: Molecular systems biology, Vol. 3, pp. 89, (2007) (PubMed).

Target Details for FBXO4

Target: FBXO4 (F-Box Protein 4 (FBXO4))

Alternative Name: FBXO4 (FBXO4 Products)

Synonyms: FBX4 antibody, 1700096C12Rik antibody, AI851261 antibody, AW494535 antibody, Fbx4 antibody, FBXO4 antibody, fbx4 antibody, si:dkey-46a10.2 antibody, F-box protein 4 antibody, F-box protein 4 L homeolog antibody, FBXO4 antibody, Fbxo4 antibody, fbxo4 antibody, fbxo4.L antibody

Background: FBXO4 is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. FBXO4 belongs to the Fbxs class.This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Alternative splicing of this gene generates 2 transcript variants.
Alias Symbols: DKFZp547N213, FBX4, FLJ10141
Protein Interaction Partner: CUL1, TERF1, SKP1, CCND1, HSP90AA1, TP53, ELAVL1, FBXO4, YWHAE, FBXW8, ELF4, UBE2D2, CDC34, NEDD8,
Protein Size: 307

Molecular Weight: 35 kDa

Gene ID: 26272

NCBI Accession: NM_033484, NP_277019

UniProt: Q9UKT5