ZCCHC11 antibody (Middle Region)

Catalog No. ABIN2774235

Product Details anti-ZCCHC11 Antibody

Target: ZCCHC11 (Zinc Finger, CCHC Domain Containing 11 (ZCCHC11))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZCCHC11 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: GSAQQQGDQS IRTRQSSECS ESPSYSPQPQ PFPQNSSQSA AITQPSSQPG

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 92%, Human: 100%, Mouse: 92%, Rabbit: 92%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against ZCCHC11. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ZCCHC11

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1645 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for ZCCHC11

Target: ZCCHC11 (Zinc Finger, CCHC Domain Containing 11 (ZCCHC11))

Alternative Name: ZCCHC11 (ZCCHC11 Products)

Background: ZCCHC11 is the uridylyltransferase that acts as a suppressor of microRNA (miRNA) biogenesis by specifically mediating the terminal uridylation of some miRNAs. ZCCHC11 catalyzes the 3' uridylation of precursor let-7 (pre-let-7), a miRNA precursor. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cells and is required for ES cells to maintain pluripotency.ZCCHC11 does not bind RNA by itself, recruited to pre-let-7 miRNAs via its interaction with LIN28A and LIN28B. ZCCHC11 also catalyzes the 3' uridylation of miR-26A, a miRNA that represses IL6 transcript, leading to abrogate IL6 transcript repression and promote cytokine expression.ZCCHC11 may also suppress Toll-like receptor-induced NF-kappa-B activity via binding to T2BP. ZCCHC11 does not play a role in replication-dependent histone mRNA degradation.
Alias Symbols: PAPD3, TUT4
Protein Interaction Partner: UBC, MYC, TIFA, CAND1, DCUN1D1, COPS5, CUL1, CUL2, CUL4B, CUL5, ELAVL1,
Protein Size: 1645

Molecular Weight: 185 kDa

Gene ID: 23318

NCBI Accession: NM_001009881, NP_001009881

UniProt: Q5TAX3

Pathways: Stem Cell Maintenance