CAMKK2 antibody (N-Term)

Catalog No. ABIN2774040

Product Details anti-CAMKK2 Antibody

Target: CAMKK2 (Calcium/calmodulin-Dependent Protein Kinase Kinase 2, beta (CAMKK2))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Pig, Guinea Pig, Cow, Rabbit, Dog, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CAMKK2 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: GGLAAGGSLD MNGRCICPSL PYSPVSSPQS SPRLPRRPTV ESHHVSITGM

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 86%, Human: 100%, Mouse: 86%, Pig: 93%, Rabbit: 93%, Rat: 86%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against CAMKK2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CAMKK2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 498 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for CAMKK2

Target: CAMKK2 (Calcium/calmodulin-Dependent Protein Kinase Kinase 2, beta (CAMKK2))

Alternative Name: CAMKK2 (CAMKK2 Products)

Background: CAMKK2 is a calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Isoform 1, isoform 2 and isoform 3 phosphorylate CAMK1 and CAMK4. Isoform 3 phosphorylates CAMK1D. Isoform 4, isoform 5 and isoform 6 lacking part of the calmodulin-binding domain are inactive. CAMKK2 seems to be involved in hippocampal activation of CREB1.The product of this gene belongs to the Serine/Threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This protein plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade by phosphorylating the downstream kinases CaMK1 and CaMK4. Seven transcript variants encoding six distinct isoforms have been identified for this gene. Additional splice variants have been described but their full-length nature has not been determined. The identified isoforms exhibit a distinct ability to undergo autophosphorylation and to phosphorylate the downstream kinases.
Alias Symbols: CAMKK, CAMKKB, KIAA0787, MGC15254
Protein Interaction Partner: HSP90AA1, APP, UBC, NEDD4, CEP63, ESYT2, FANCI, OBSL1, ATG4B, XPOT, GCN1L1, IQGAP2, SMC3, SLC25A11, SMC1A, PRKDC, PRKAG1, PRKAA2, IRAK1, FLNC, FLNA, MAPK14, CALM1, CAMK4, CAMK1, PRKACA, SGK1,
Protein Size: 498

Molecular Weight: 55 kDa

Gene ID: 10645

NCBI Accession: NM_153500, NP_705720

UniProt: Q96RR4

Pathways: AMPK Signaling