WARS2 antibody (Middle Region)

Catalog No. ABIN2774013

Product Details anti-WARS2 Antibody

Target: WARS2 (Tryptophanyl tRNA Synthetase 2, Mitochondrial (WARS2))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Saccharomyces cerevisiae, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WARS2 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: TTKQKHDGTV GLLTYPVLQA ADILLYKSTH VPVGEDQVQH MELVQDLAQG

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 93%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against WARS2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human WARS2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 220 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-WARS2 antibody (ABIN2774013)

Wang, Sips, Khin, Rotival, Sun, Ahmed, Widjaja, Schafer, Yusoff, Choksi, Ko, Singh, Epstein, Guan, Houštěk, Mracek, Nuskova, Mikell, Tan, Pesce, Kolar, Bottolo, Mancini, Hubner, Pravenec, Petretto et al.: "Wars2 is a determinant of angiogenesis. ..." in: Nature communications, Vol. 7, pp. 12061, (2016) (PubMed).

Target Details for WARS2

Target: WARS2 (Tryptophanyl tRNA Synthetase 2, Mitochondrial (WARS2))

Alternative Name: WARS2 (WARS2 Products)

Synonyms: 5730427B17Rik antibody, 9430020O07Rik antibody, AI413375 antibody, TrpRS antibody, zgc:110828 antibody, tryptophanyl tRNA synthetase 2, mitochondrial antibody, tryptophanyl tRNA synthetase 2 (mitochondrial) antibody, WARS2 antibody, Wars2 antibody, wars2 antibody

Background: Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. WARS2 is the mitochondrial tryptophanyl-tRNA synthetase. Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. This gene encodes the mitochondrial tryptophanyl-tRNA synthetase. Two alternative transcripts encoding different isoforms have been described.
Alias Symbols: TrpRS
Protein Interaction Partner: UBC, APP,
Protein Size: 220

Molecular Weight: 24 kDa

Gene ID: 10352

NCBI Accession: NM_201263, NP_957715

UniProt: Q9UGM6