PNKP antibody (N-Term)

Catalog No. ABIN2774011

Product Details anti-PNKP Antibody

Target: PNKP (Polynucleotide Kinase 3'-Phosphatase (PNKP))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PNKP antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: MGEVEAPGRL WLESPPGGAP PIFLPSDGQA LVLGRGPLTQ VTDRKCSRTQ

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against PNKP. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PNKP

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 521 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for PNKP

Target: PNKP (Polynucleotide Kinase 3'-Phosphatase (PNKP))

Alternative Name: PNKP (PNKP Products)

Background: This locus represents a gene involved in DNA repair. In response to ionizing radiation or oxidative damage, the protein encoded by this locus catalyzes 5' phosphorylation and 3' dephosphorylation of nucleic acids. Mutations at this locus have been associated with microcephaly, seizures, and developmental delay.
Alias Symbols: PNK, MCSZ, EIEE10
Protein Interaction Partner: ZNF639, IKZF1, TNIP1, ZBTB14, RPA3, RPA2, RPA1, EED, rev, KDM1A, SUV39H1, HNRNPU, GZMK, XRCC6, CDK11B, PARP1, MCM3, SMAD5, LIG4, XRCC1, TCOF1, SSRP1, PRKDC, NRD1, NASP, MCM5, C18orf25, UBE2O, GEMIN4, AGTPBP1, PNKP, SUPT16H, VPRBP, NOLC1, TRIM26, XRCC5, XR
Protein Size: 521

Molecular Weight: 57 kDa

Gene ID: 11284

NCBI Accession: NM_007254, NP_009185

UniProt: Q96T60

Pathways: DNA Damage Repair, Nucleotide Phosphorylation