FZR1 antibody (N-Term)

Catalog No. ABIN2773984

Product Details anti-FZR1 Antibody

Target: FZR1 (Fizzy/cell Division Cycle 20 Related 1 (FZR1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Zebrafish (Danio rerio), Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FZR1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: RQIIIQNENT VPCVSEMRRT LTPANSPVSS PSKHGDRFIP SRAGANWSVN

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 93%, Pig: 100%, Rat: 93%, Zebrafish: 92%

Characteristics: This is a rabbit polyclonal antibody against Fzr1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide corresponding to a region of Mouse

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 493 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for FZR1

Target: FZR1 (Fizzy/cell Division Cycle 20 Related 1 (FZR1))

Alternative Name: Fzr1 (FZR1 Products)

Synonyms: CDH1-C antibody, FYR antibody, FZR antibody, CDH1 antibody, fzr1 antibody, CDC20C antibody, FZR2 antibody, HCDH antibody, HCDH1 antibody, AW108046 antibody, Cdh1 antibody, Fyr antibody, zgc:55450 antibody, fizzy and cell division cycle 20 related 1 antibody, fizzy/cell division cycle 20 related 1 antibody, fizzy-related protein homolog antibody, fizzy/cell division cycle 20 related 1 (Drosophila) antibody, fizzy/cell division cycle 20 related 1a antibody, Fzr1 antibody, CDH1-C antibody, FZR1 antibody, LOC100027495 antibody, LOC100179951 antibody, fzr1a antibody

Background: Fzr1 is the key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C), which confers substrate specificity upon the complex. Fzr1 associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis.
Alias Symbols: AW108046, Cdh1, FZR, FZR2, Fyr, HCDH, HCDH1
Protein Interaction Partner: Arhgap35, Rrm2, Ccnb1, Cdc27, Sirt2, Smurf1, Skil, Ube2c, Csf3r,
Protein Size: 493

Molecular Weight: 55 kDa

Gene ID: 56371

NCBI Accession: NM_019757, NP_062731

UniProt: Q9R1K5

Pathways: DNA Replication, Synthesis of DNA