HLA-F antibody (N-Term)

Catalog No. ABIN2773941

Product Details anti-HLA-F Antibody

Target: HLA-F (HLAF) (HLA Class I alpha F (HLAF))

Binding Specificity: N-Term

Reactivity: Human, Pig, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HLA-F antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: EAGSHTLQGM NGCDMGPDGR LLRGYHQHAY DGKDYISLNE DLRSWTAADT

Predicted Reactivity: Cow: 93%, Human: 100%, Pig: 100%

Characteristics: This is a rabbit polyclonal antibody against HLA-F. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human HLA-F

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 442 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for HLA-F

Target: HLA-F (HLAF) (HLA Class I alpha F (HLAF))

Alternative Name: HLA-F (HLAF Products)

Synonyms: HLAF antibody, CDA12 antibody, HLA-5.4 antibody, HLA-CDA12 antibody, major histocompatibility complex, class I, F antibody, HLA-F antibody

Background: HLA-F belongs to the HLA class I heavy chain paralogues. It is a non-classical heavy chain that forms a heterodimer with a beta-2 microglobulin light chain, with the heavy chain anchored in the membrane. Unlike most other HLA heavy chains, this molecule is localized in the endoplasmic reticulum and Golgi apparatus, with a small amount present at the cell surface in some cell types. It contains a divergent peptide-binding groove, and is thought to bind a restricted subset of peptides for immune presentation. This gene belongs to the HLA class I heavy chain paralogues. It encodes a non-classical heavy chain that forms a heterodimer with a beta-2 microglobulin light chain, with the heavy chain anchored in the membrane. Unlike most other HLA heavy chains, this molecule is localized in the endoplasmic reticulum and Golgi apparatus, with a small amount present at the cell surface in some cell types. It contains a divergent peptide-binding groove, and is thought to bind a restricted subset of peptides for immune presentation. This gene exhibits few polymorphisms. Multiple transcript variants encoding different isoforms have been found for this gene. These variants lack a coding exon found in transcripts from other HLA paralogues due to an altered splice acceptor site, resulting in a shorter cytoplasmic domain.
Alias Symbols: CDA12, HLA-5.4, HLA-CDA12, HLAF
Protein Interaction Partner: UBC, LILRB2, LILRB1, TAP1, HLA-F, B2M,
Protein Size: 442

Molecular Weight: 48 kDa

Gene ID: 3134

NCBI Accession: NM_001098479, NP_001091949

UniProt: P30511

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Human Leukocyte Antigen (HLA) in Adaptive Immune Response