ME3 antibody (N-Term)

Catalog No. ABIN2773921

Product Details anti-ME3 Antibody

Target: ME3 (Malic Enzyme 3, NADP(+)-Dependent, Mitochondrial (ME3))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit, Zebrafish (Danio rerio), Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ME3 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: PGPARPVPLK KRGYDVTRNP HLNKGMAFTL EERLQLGIHG LIPPCFLSQD

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 92%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against ME3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ME3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 604 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for ME3

Target: ME3 (Malic Enzyme 3, NADP(+)-Dependent, Mitochondrial (ME3))

Alternative Name: ME3 (ME3 Products)

Synonyms: NADP-ME antibody, 1700020C08Rik antibody, B230207H15Rik antibody, im:7151680 antibody, wu:fi43b04 antibody, zgc:163117 antibody, malic enzyme 3 antibody, malic enzyme 3, NADP(+)-dependent, mitochondrial antibody, malic enzyme 3, NADP(+)-dependent, mitochondrial L homeolog antibody, NADP malic enzyme 3 antibody, ME3 antibody, Me3 antibody, me3 antibody, me3.L antibody

Background: Malic enzyme catalyzes the oxidative decarboxylation of malate to pyruvate using either NAD+ or NADP+ as a cofactor. Mammalian tissues contain 3 distinct isoforms of malic enzyme: a cytosolic NADP(+)-dependent isoform, a mitochondrial NADP(+)-dependent isoform, and a mitochondrial NAD(+)-dependent isoform. ME3 is a mitochondrial NADP(+)-dependent isoform. Malic enzyme catalyzes the oxidative decarboxylation of malate to pyruvate using either NAD+ or NADP+ as a cofactor. Mammalian tissues contain 3 distinct isoforms of malic enzyme: a cytosolic NADP(+)-dependent isoform, a mitochondrial NADP(+)-dependent isoform, and a mitochondrial NAD(+)-dependent isoform. This gene encodes a mitochondrial NADP(+)-dependent isoform. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.
Alias Symbols: FLJ34862, NADP-ME
Protein Interaction Partner: UBC, TRAF4,
Protein Size: 604

Molecular Weight: 67 kDa

Gene ID: 10873

NCBI Accession: NM_001014811, NP_001014811

UniProt: Q16798

Pathways: Warburg Effect