BHMT antibody (N-Term)

Catalog No. ABIN2773785

Product Details anti-BHMT Antibody

Target: BHMT (Betaine--Homocysteine S-Methyltransferase (BHMT))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Horse, Guinea Pig, Rabbit, Cow, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BHMT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: AVEHPEAVRQ LHREFLRAGS NVMQTFTFYA SEDKLENRGN YVLEKISGQE

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against BHMT. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human BHMT

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 406 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-BHMT antibody (ABIN2773785)

Jiang, Hurt, Breen, Stabler, Allen, Orlicky, Maclean: "Sex-specific dysregulation of cysteine oxidation and the methionine and folate cycles in female cystathionine gamma-lyase null mice: a serendipitous model of the methylfolate trap." in: Biology open, Vol. 4, Issue 9, pp. 1154-62, (2015) (PubMed).

Hu, Lausted, Yoo, Yan, Brightman, Chen, Wang, Bu, Hood: "Quantitative liver-specific protein fingerprint in blood: a signature for hepatotoxicity." in: Theranostics, Vol. 4, Issue 2, pp. 215-28, (2014) (PubMed).

Maclean, Jiang, Greiner, Allen, Stabler et al.: "Long-term betaine therapy in a murine model of cystathionine beta-synthase deficient homocystinuria: decreased efficacy over time reveals a significant threshold effect between elevated homocysteine ..." in: Molecular genetics and metabolism, Vol. 105, Issue 3, pp. 395-403, (2012) (PubMed).

Target Details for BHMT

Target: BHMT (Betaine--Homocysteine S-Methyltransferase (BHMT))

Alternative Name: BHMT (BHMT Products)

Synonyms: BHMT1 antibody, hm:zehn2153 antibody, wu:fb53h01 antibody, wu:fb63c08 antibody, wu:fj64d01 antibody, zgc:123027 antibody, betaine--homocysteine S-methyltransferase antibody, betaine--homocysteine S-methyltransferase L homeolog antibody, betaine-homocysteine methyltransferase antibody, betaine-homocysteine S-methyltransferase antibody, BHMT antibody, bhmt.L antibody, Bhmt antibody, bhmt antibody

Background: BHMT is a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in its gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed.Betaine-homocysteine methyltransferase is a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in BHMT could lead to hyperhomocyst(e)inemia,but such a defect has not yet been observed.
Alias Symbols: BHMT1
Protein Interaction Partner: BHMT, BBS4, BBS2, BBS1, ALG9, LAMTOR3,
Protein Size: 406

Molecular Weight: 45 kDa

Gene ID: 635

NCBI Accession: NM_001713, NP_001704

UniProt: Q93088

Pathways: Methionine Biosynthetic Process