PIWIL1 antibody (N-Term)

Catalog No. ABIN2773782

Product Details anti-PIWIL1 Antibody

Target: PIWIL1 (Piwi-Like 1 (PIWIL1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIWIL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: FQELSLAERG GRRRDFHDLG VNTRQNLDHV KESKTGSSGI IVRLSTNHFR

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 77%

Characteristics: This is a rabbit polyclonal antibody against PIWIL1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PIWIL1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 861 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-PIWIL1 antibody (ABIN2773782)

Tarabay, Achour, Teletin, Ye, Teissandier, Mark, Bourchis, Viville: "Tex19 paralogs are new members of the piRNA pathway controlling retrotransposon suppression." in: Journal of cell science, Vol. 130, Issue 8, pp. 1463-1474, (2017) (PubMed).

Sugimoto, Kage, Aki, Sano, Kitagawa, Nagase, Yatomi, Ohishi, Takai: "The induction of H3K9 methylation by PIWIL4 at the p16Ink4a locus." in: Biochemical and biophysical research communications, Vol. 359, Issue 3, pp. 497-502, (2007) (PubMed).

Target Details for PIWIL1

Target: PIWIL1 (Piwi-Like 1 (PIWIL1))

Alternative Name: PIWIL1 (PIWIL1 Products)

Synonyms: ziwi antibody, PIWI antibody, HIWI antibody, MIWI antibody, piwi like RNA-mediated gene silencing 1 antibody, PIWI-like protein 1 antibody, putative PIWI-like protein 1 antibody, putative argonaut-like protein antibody, piwi-like RNA-mediated gene silencing 1 antibody, PIWIL1 antibody, Tb10.70.5520 antibody, LINJ_21_0470 antibody, LBRM_21_0470 antibody, piwil1 antibody, Piwil1 antibody

Background: PIWIL1 is a member of the PIWI subfamily of Argonaute proteins, evolutionarily conserved proteins containing both PAZ and Piwi motifs that play important roles in stem cell self-renewal, RNA silencing, and translational regulation in diverse organisms. PI
Alias Symbols: HIWI, PIWI, MIWI
Protein Interaction Partner: UBC, DICER1, TP63, TP73, TP53,
Protein Size: 861

Molecular Weight: 98 kDa

Gene ID: 9271

NCBI Accession: NM_004764, NP_004755

UniProt: Q96J94