CD180 antibody

Catalog No. ABIN2749219

Product Details anti-CD180 Antibody

Target: CD180 (CD180 Molecule (CD180))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD180 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies (Func)

Specificity: The mouse monoclonal antibody G28-8 recognizes an extracellular epitope of CD180, a 95-105 kDa TLR-like glycoprotein expressed on peripheral blood monocytes and dendritic cells, mantle zone B cells and marginal zone B cells, but very weakly on germinal center B cells.

Cross-Reactivity (Details): Human

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Endotoxin Level: Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

Immunogen: Human tonsillar B cells

Clone: G28-8

Isotype: IgG1 kappa

Application Details

Application Notes: Functional application: Induction of B cell proliferation.
Flow cytometry: Recommended dilution: 2 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CD180 antibody (ABIN2749219)

Olson, Graves, Shu, Ryan, Clark: "Caspase activity is required for stimulated B lymphocytes to enter the cell cycle." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 12, pp. 6065-72, (2003) (PubMed).

Clark, Shu, Lüscher, Draves, Banchereau, Ledbetter, Valentine: "Activation of human B cells. Comparison of the signal transduced by IL-4 to four different competence signals." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 143, Issue 12, pp. 3873-80, (1990) (PubMed).

Valentine, Clark, Shu, Norris, Ledbetter: "Antibody to a novel 95-kDa surface glycoprotein on human B cells induces calcium mobilization and B cell activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 140, Issue 12, pp. 4071-8, (1988) (PubMed).

Target Details for CD180

Target: CD180 (CD180 Molecule (CD180))

Alternative Name: CD180 (CD180 Products)

Background: CD180 Molecule,CD180, also known as RP105 (or Bgp95, LY64) is a type I membrane glycoprotein of Toll-like receptor (TLR) family. Its cytoplasmic tail is short and unlike the TLRs, it lacks the TIR domain. CD180 expression is dependent on the coexpression of its helper molecule, MD-1, and mirrors that of TLR4 on antigen-presenting cells. CD180 regulates recognition of LPS and signaling in B cells, via interacting directly with the TLR4 signaling complex, inhibiting its ability to bind microbial ligands. Ligation of CD180 by monoclonal antibodies leads to B cell activation, upregulation of CD80/CD86, and increase in cell size.,LY64, Ly78, RP105, Bgp95

Gene ID: 4064

UniProt: Q99467

Pathways: Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades