CD82 antibody (APC)

Catalog No. ABIN2749208

Product Details anti-CD82 Antibody (APC)

Target: CD82

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD82 antibody is conjugated to APC

Application: Flow Cytometry (FACS)

Specificity: The mouse monoclonal antibody C33 recognizes an extracellular/luminal epitope of CD82, a widely expressed cell surface protein of the tetraspanin family. CD82 is also found in endosome/lysosome compartments.

Cross-Reactivity (Details): Human, Other not tested

Purification: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: C91/PL (human HTLV-1+ T cell line)

Clone: C33

Isotype: IgG2a

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Comment:

The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Restrictions: For Research Use only

Handling

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References for anti-CD82 antibody (APC) (ABIN2749208)

Schatzlmaier, Supper, Göschl, Zwirzitz, Eckerstorfer, Ellmeier, Huppa, Stockinger: "Rapid multiplex analysis of lipid raft components with single-cell resolution." in: Science signaling, Vol. 8, Issue 395, pp. rs11, (2015) (PubMed).

Escola, Kleijmeer, Stoorvogel, Griffith, Yoshie, Geuze: "Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B-lymphocytes." in: The Journal of biological chemistry, Vol. 273, Issue 32, pp. 20121-7, (1998) (PubMed).

Ueda, Ichikawa, Tamaru, Mikata, Akakura, Akimoto, Imai, Yoshie, Shiraishi, Yatani, Ito, Shimazaki: "Expression of the KAI1 protein in benign prostatic hyperplasia and prostate cancer." in: The American journal of pathology, Vol. 149, Issue 5, pp. 1435-40, (1996) (PubMed).

Imai, Kakizaki, Nishimura, Yoshie: "Molecular analyses of the association of CD4 with two members of the transmembrane 4 superfamily, CD81 and CD82." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 3, pp. 1229-39, (1995) (PubMed).

Imai, Yoshie et al.: "C33 antigen and M38 antigen recognized by monoclonal antibodies inhibitory to syncytium formation by human T cell leukemia virus type 1 are both members of the transmembrane 4 superfamily and ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 11, pp. 6470-81, (1994) (PubMed).

Fukudome, Furuse, Imai, Nishimura, Takagi, Hinuma, Yoshie et al.: "Identification of membrane antigen C33 recognized by monoclonal antibodies inhibitory to human T-cell leukemia virus type 1 (HTLV-1)-induced syncytium formation: altered glycosylation of C33 antigen ..." in: Journal of virology, Vol. 66, Issue 3, pp. 1394-401, (1992) (PubMed).

Target Details for CD82

Target: CD82

Alternative Name: CD82 (CD82 Products)

Synonyms: AA682076 antibody, AL023070 antibody, C33 antibody, IA4 antibody, Kai1 antibody, Tspan27 antibody, 4F9 antibody, GR15 antibody, KAI1 antibody, R2 antibody, SAR2 antibody, ST6 antibody, TSPAN27 antibody, CD82 antigen antibody, CD82 molecule antibody, Cd82 molecule antibody, Cd82 antibody, CD82 antibody

Background: CD82 Molecule,CD82 (KAI1), a member of the tetraspanin family, forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules. These complexes influence adhesion, morphology, activation, proliferation and differentiation of B, T and other cells. CD82 regulates cytoskeleton rearrangement and may participate in the turnover of the tetraspanin complex members. Besides in the plasma membrane, CD82 is localized also in endosome/lysosome compartments. Tumour-suppressive roles of CD82 have been demonstrated.,R2, 4F9, C33, IA4, ST6, GR15, KAI1, SAR2, TSPAN27

Gene ID: 3732

UniProt: P27701

Pathways: p53 Signaling