PVRL2 antibody (PE)

Catalog No. ABIN2749199

Product Details anti-PVRL2 Antibody (PE)

Target: PVRL2 (Poliovirus Receptor-Related 2 (Herpesvirus Entry Mediator B) (PVRL2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PVRL2 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Specificity: The mouse monoclonal antibody R2.525 recognizes an extracellular epitope on CD112, a type I transmembrane glycoprotein expressed by myelomonocytic and megakaryocytic cells, and by CD34+ hematopoietic progenitors.

Cross-Reactivity (Details): Human

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: NIH/3T3 cells transfected with human Nectin-2

Clone: R2-525

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Comment:

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Restrictions: For Research Use only

Handling

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References for anti-PVRL2 antibody (PE) (ABIN2749199)

Hou, Ge, Zheng, Wei, Sun, Tian: "CD226 protein is involved in immune synapse formation and triggers Natural Killer (NK) cell activation via its first extracellular domain." in: The Journal of biological chemistry, Vol. 289, Issue 10, pp. 6969-77, (2014) (PubMed).

Enqvist, Nilsonne, Hammarfjord, Wallin, Björkström, Björnstedt, Hjerpe, Ljunggren, Dobra, Malmberg, Carlsten: "Selenite induces posttranscriptional blockade of HLA-E expression and sensitizes tumor cells to CD94/NKG2A-positive NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 187, Issue 7, pp. 3546-54, (2011) (PubMed).

Target Details for PVRL2

Target: PVRL2 (Poliovirus Receptor-Related 2 (Herpesvirus Entry Mediator B) (PVRL2))

Alternative Name: CD112 (PVRL2 Products)

Synonyms: prr2 antibody, AI325026 antibody, AI987993 antibody, Cd112 antibody, MPH antibody, Pvr antibody, Pvs antibody, nectin-2 antibody, CD112 antibody, HVEB antibody, PRR2 antibody, PVRR2 antibody, nectin cell adhesion molecule 2 antibody, Nectin2 antibody, NECTIN2 antibody

Background: Nectin cell adhesion molecule 2,CD112, also known as nectin-2, is a transmembrane glycoprotein involved in organization of adherens junctions. It also serves as a target molecule for entry of certain strains of herpes simplex virus (HSV) and pseudorabies virus (PRV). It is homologous to CD155, which serves as a target molecule for polio virus. CD112 seems to play a role in neural tube formation, with N-cadherin. Inside the cell, CD112 is connected with actin cytoskeleton through afadin. Variations in the CD112 gene have been associated with differences in the severity of multiple sclerosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.,NECTIN2, HVEB, PRR2, PVRL2, PVRR2

Gene ID: 5819

UniProt: Q92692

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization