CCR3 antibody (PE)

Catalog No. ABIN2749177

Product Details anti-CCR3 Antibody (PE)

Target: CCR3 (Chemokine (C-C Motif) Receptor 3 (CCR3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CCR3 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Specificity: The mouse monoclonal antibody 5E8 recognizes an extracellular epitope of CD193 (chemokine receptor 3), an approximately 41 kDa protein expressed above all in eosinophils and basophils.

Cross-Reactivity (Details): Human

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: human CD193 transfectants

Clone: 5E8

Isotype: IgG2b kappa

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Comment:

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Restrictions: For Research Use only

Handling

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References for anti-CCR3 antibody (PE) (ABIN2749177)

Morshed, Hlushchuk, Simon, Walls, Obata-Ninomiya, Karasuyama, Djonov, Eggel, Kaufmann, Simon, Yousefi: "NADPH oxidase-independent formation of extracellular DNA traps by basophils." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 192, Issue 11, pp. 5314-23, (2014) (PubMed).

Target Details for CCR3

Target: CCR3 (Chemokine (C-C Motif) Receptor 3 (CCR3))

Alternative Name: CD193 (CCR3 Products)

Synonyms: CC-CKR-3 antibody, CD193 antibody, CKR3 antibody, CMKBR3 antibody, CC-CKR3 antibody, Cmkbr1l2 antibody, Cmkbr3 antibody, C-C motif chemokine receptor 3 antibody, chemokine (C-C motif) receptor 3 antibody, CCR3 antibody, Ccr3 antibody

Background: C-C motif chemokine receptor 3,CD193 / CCR3 is a G-protein coupled receptor for several chemokines, namely CCL11 (eotaxin), CCL26 (eotaxin-3), CCL7 (MCP-4), or CCL5 (RANTES). It is highly expressed on eosinophils and basophils, and is also detected in TH1 and TH2 cells, as well as in airway epithelial cells. CD193 is the key eosinophil chemokine receptor responsible for regulation of eosinophil migration and function. This receptor may contribute to the accumulation and activation of eosinophils and other inflammatory cells in the allergic airway. It is also known to be an entry co-receptor for HIV-1.,CCR3, CKR3, CMKBR3, CC-CKR-3

Gene ID: 1232

UniProt: P51677