CD42b antibody (PE)

Catalog No. ABIN2749168

Product Details anti-CD42b Antibody (PE)

Target: CD42b (GP1BA) (Glycoprotein Ib (Platelet), alpha Polypeptide (GP1BA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD42b antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Specificity: The mouse monoclonal antibody AK2 recognizes an extracellular epitope of CD42b (GPIb alpha), a 135-145 kDa membrane glycoprotein expressed on platelets and megakaryocytes. CD42b and CD42c (GPIb beta) are composed in a disulfide linked heterodimer (CD42b/c, 160 kDa), CD42b/c forms a noncovalent complex with CD42a and CD42d.

Cross-Reactivity (Details): Human

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Human platelets

Clone: AK2

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Comment:

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Restrictions: For Research Use only

Handling

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References for anti-CD42b antibody (PE) (ABIN2749168)

Welsh, Colace, Muthard, Stalker, Brass, Diamond: "Platelet-targeting sensor reveals thrombin gradients within blood clots forming in microfluidic assays and in mouse." in: Journal of thrombosis and haemostasis : JTH, Vol. 10, Issue 11, pp. 2344-53, (2014) (PubMed).

Vettore, Scandellari, Moro, Lombardi, Scapin, Randi, Fabris et al.: "Novel point mutation in a leucine-rich repeat of the GPIbalpha chain of the platelet von Willebrand factor receptor, GPIb/IX/V, resulting in an inherited dominant form of Bernard-Soulier syndrome ..." in: Haematologica, Vol. 93, Issue 11, pp. 1743-7, (2008) (PubMed).

Target Details for CD42b

Target: CD42b (GP1BA) (Glycoprotein Ib (Platelet), alpha Polypeptide (GP1BA))

Alternative Name: CD42b (GP1BA Products)

Background: Glycoprotein Ib platelet subunit alpha,CD42b (GPIb alpha) composes together with GPIb beta, GPIX and GPV the GPIb-IX-V receptor complex critical in the process of platelet-rich thrombus formation by tethering the platelet to a thrombogenic surface. CD42b binds to von Willebrand factor (VWF) exposed at a site of vascular injury, as well as to thrombin, coagulation factors XI and XII, high molecular weight kininogen, TSP-1, integrin Mac-1 and P-selectin. The extracellular domain of CD42b by its interactions also contributes to metastasis.,BSS, GP1B, VWDP, CD42B, GPIbA, BDPLT1, BDPLT3, DBPLT3

Gene ID: 2811

UniProt: P07359