Notch1 antibody (APC)

Catalog No. ABIN2749161

Product Details anti-Notch1 Antibody (APC)

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Notch1 antibody is conjugated to APC

Application: Flow Cytometry (FACS)

Specificity: The mouse monoclonal antibody mN1A recognizes intracellular domain of Notch 1 protein, mainly its activated form. The unprocessed Notch 1 protein is recognized with lower affinity.

No Cross-Reactivity: Rat

Cross-Reactivity (Details): Human, Mouse

Purification: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: GST fusion protein containing cdc10-NCR region of mouse Notch1

Clone: MN1A

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-5 μg/mL. Intracellular staining.

Comment:

The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References for anti-Notch1 antibody (APC) (ABIN2749161)

Khwaja, Liu, Tong, Jin, Pear, van Deursen, Bram: "HIV-1 Rev-binding protein accelerates cellular uptake of iron to drive Notch-induced T cell leukemogenesis in mice." in: The Journal of clinical investigation, Vol. 120, Issue 7, pp. 2537-48, (2010) (PubMed).

Kefas, Comeau, Floyd, Seleverstov, Godlewski, Schmittgen, Jiang, diPierro, Li, Chiocca, Lee, Fine, Abounader, Lawler, Purow: "The neuronal microRNA miR-326 acts in a feedback loop with notch and has therapeutic potential against brain tumors." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 29, Issue 48, pp. 15161-8, (2009) (PubMed).

Watanabe, Nagaoka, Lee, Bianco, Gonzales, Castro, Rangel, Sakamoto, Sun, Callahan, Salomon: "Enhancement of Notch receptor maturation and signaling sensitivity by Cripto-1." in: The Journal of cell biology, Vol. 187, Issue 3, pp. 343-53, (2009) (PubMed).

Tagami, Okochi, Yanagida, Ikuta, Fukumori, Matsumoto, Ishizuka-Katsura, Nakayama, Itoh, Jiang, Nishitomi, Kamino, Morihara, Hashimoto, Tanaka, Kudo, Chiba, Takeda: "Regulation of Notch signaling by dynamic changes in the precision of S3 cleavage of Notch-1." in: Molecular and cellular biology, Vol. 28, Issue 1, pp. 165-76, (2007) (PubMed).

Sun, Li, Vercherat, Gulbagci, Acharjee, Li, Chung, Thin, Taneja: "Stra13 regulates satellite cell activation by antagonizing Notch signaling." in: The Journal of cell biology, Vol. 177, Issue 4, pp. 647-57, (2007) (PubMed).

Huppert, Le, Schroeter, Mumm, Saxena, Milner, Kopan: "Embryonic lethality in mice homozygous for a processing-deficient allele of Notch1." in: Nature, Vol. 405, Issue 6789, pp. 966-70, (2000) (PubMed).

Target Details for Notch1

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Alternative Name: Notch 1 (NOTCH1 Products)

Synonyms: notch1 antibody, NOTCH1 antibody, hn1 antibody, tan1 antibody, notch antibody, xotch antibody, xnotch antibody, notch-1 antibody, xnotch1 antibody, TAN1 antibody, hN1 antibody, 9930111A19Rik antibody, Mis6 antibody, N1 antibody, Tan1 antibody, lin-12 antibody, NOTCH antibody, Xotch antibody, notch1-a antibody, notch 1 antibody, fibropellin-1 antibody, Notch homolog 1, translocation-associated (Drosophila) antibody, Notch homolog 1, translocation-associated antibody, notch 1 S homeolog antibody, notch1 antibody, LOC100631526 antibody, NOTCH1 antibody, Notch1 antibody, notch1.S antibody

Background: Notch receptor 1,Notch 1 is a 270-300 kDa transmembrane heterodimeric protein with multiple extracellular growth factor-like repeats, and with an intracellular domain consisting of multiple different domain types. It serves as a receptor for membrane ligands, such as Delta 1, Jagged 1 (CD339), and Jagged 2, and regulates cell fate decisions. Upon ligand binding the transmembrane form of Notch 1 is repeatedly cleaved to provide approximately 120 kDa Notch intracellular fragment (NICD), which translocates to the nucleus and acts as a part of transcriptional complexes that alter differentiation, proliferation, and apoptosis. The highest level of Notch 1 expression is in brain, lung and thymus.,AOS5, TAN1, hN1, AOVD1

Gene ID: 4851

UniProt: P46531

Pathways: Notch Signaling, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development