NGFR antibody (APC)

Catalog No. ABIN2749123

Product Details anti-NGFR Antibody (APC)

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Reactivity: Human, Non-Human Primate, Rabbit, Cat, Ferret

Host: Mouse

Clonality: Monoclonal

Conjugate: This NGFR antibody is conjugated to APC

Application: Flow Cytometry (FACS)

Specificity: The mouse monoclonal antibody NGFR5 (originally C34C) recognizes an epitope within ammino acids 1 - 160 of CD271/NGFR, a 75 kDa transmembrane glycoprotein of the TNFR superfamily.

No Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Human, Non-Human Primates, Feline (Cat), Rabbit, Ferret

Purification: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Purified CD271 protein isolated from human melanoma cell line A875

Clone: NGFR5

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Extracellular and intracellular staining.

Comment:

The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Restrictions: For Research Use only

Handling

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References for anti-NGFR antibody (APC) (ABIN2749123)

Alpers, Hudkins, Ferguson, Johnson, Schatteman, Bothwell: "Nerve growth factor receptor expression in fetal, mature, and diseased human kidneys." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 69, Issue 6, pp. 703-13, (1994) (PubMed).

Thompson, Schatteman, Gown, Bothwell: "A monoclonal antibody against nerve growth factor receptor. Immunohistochemical analysis of normal and neoplastic human tissue." in: American journal of clinical pathology, Vol. 92, Issue 4, pp. 415-23, (1989) (PubMed).

Schatteman, Gibbs, Lanahan, Claude, Bothwell: "Expression of NGF receptor in the developing and adult primate central nervous system." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 8, Issue 3, pp. 860-73, (1988) (PubMed).

Marano, Dietzschold, Earley, Schatteman, Thompson, Grob, Ross, Bothwell, Atkinson, Koprowski: "Purification and amino terminal sequencing of human melanoma nerve growth factor receptor." in: Journal of neurochemistry, Vol. 48, Issue 1, pp. 225-32, (1987) (PubMed).

Target Details for NGFR

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Alternative Name: CD271 (NGFR Products)

Synonyms: ngfr antibody, wu:fb38c02 antibody, wu:fc10h01 antibody, CD271 antibody, Gp80-LNGFR antibody, TNFRSF16 antibody, p75(NTR) antibody, p75NTR antibody, LNGFR antibody, RNNGFRR antibody, Tnfrsf16 antibody, p75 antibody, NRH1b antibody, fullback antibody, nrh1 antibody, p75ntr antibody, tnfrsf16 antibody, p75NGFR antibody, nerve growth factor receptor antibody, neurotrophin receptor associated death domain antibody, nerve growth factor receptor S homeolog antibody, nerve growth factor receptor (TNFR superfamily, member 16) antibody, NGFR antibody, nradd antibody, Ngfr antibody, ngfr.S antibody

Background: Nerve growth factor receptor,CD271 / NGFR, also known as p75NGFR or p75NTR, is a 75 kDa low affinity receptor for the NGF (nerve growth factor), BDNF (brain-derived growth factor), and other neurotrophins, such as NT3 and NT4/5. Unlike other members of the tumor necrosis factor receptor superfamily of transmembrane proteins, CD271 has unique intracellular domain structure (lacks catalytic activity) and downstream signaling partners. Triggered by its ligands CD271 affects growth, differentiation, migration and death of the nervous system cells.,TNFRSF16, NGFR, p75NTR, p75NGFR

Gene ID: 4804

UniProt: P08138

Pathways: NF-kappaB Signaling, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Growth Factor Binding