CD235a/GYPA antibody (PE)

Catalog No. ABIN2749119

Product Details anti-CD235a/GYPA Antibody (PE)

Target: CD235a/GYPA (GYPA) (Glycophorin A (GYPA))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD235a/GYPA antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Specificity: The mouse monoclonal antibody JC159 recognizes an epitope between amino acids 27 and 40 of the extracellular portion of CD235a (glycophorin A), a sialoglycoprotein expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL. The antibody does not react with glycophorin B.

Cross-Reactivity (Details): Human, Rat

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Membrane preparation from splenic hairy cell leukemia

Clone: JC159

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Comment:

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Restrictions: For Research Use only

Handling

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References for anti-CD235a/GYPA antibody (PE) (ABIN2749119)

Yamauchi, Takenaka, Urata, Shima, Kikushige, Tokuyama, Iwamoto, Nishihara, Iwasaki, Miyamoto, Honma, Nakao, Matozaki, Akashi: "Polymorphic Sirpa is the genetic determinant for NOD-based mouse lines to achieve efficient human cell engraftment." in: Blood, Vol. 121, Issue 8, pp. 1316-25, (2013) (PubMed).

Beck, Jagodzinski, Eller, Thelian, Matyas, Kunz, Alving: "Platelets and erythrocyte-bound platelets bind infectious HIV-1 in plasma of chronically infected patients." in: PLoS ONE, Vol. 8, Issue 11, pp. e81002, (2013) (PubMed).

Alijotas-Reig, Palacio-Garcia, Llurba, Vilardell-Tarres: "Cell-derived microparticles and vascular pregnancy complications: a systematic and comprehensive review." in: Fertility and sterility, Vol. 99, Issue 2, pp. 441-9, (2013) (PubMed).

Maijenburg, Kleijer, Vermeul, Mul, van Alphen, van der Schoot, Voermans: "The composition of the mesenchymal stromal cell compartment in human bone marrow changes during development and aging." in: Haematologica, Vol. 97, Issue 2, pp. 179-83, (2012) (PubMed).

Target Details for CD235a/GYPA

Target: CD235a/GYPA (GYPA) (Glycophorin A (GYPA))

Alternative Name: CD235a (GYPA Products)

Background: Glycophorin A (MNS blood group),CD235a (glycophorin A, GPA) is a transmembrane sialoglycoprotein expressed on erythrocytes and their precursors. Similarly to CD235b (glycophorin B, GPB), these molecules provide the cells with a large mucin-like surface, which minimalizes aggregation between erythrocytes in the circulation. GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities. CD235a is a receptor of Hsa, a Streptococcus adhesin.,Glycophorin A, GYPA, GPA, PAS-2, Sialoglycoprotein alpha, MN sialoglycoprotein, GPSAT, GPErik, MSN blood group

Gene ID: 2993

UniProt: P02724

Pathways: Maintenance of Protein Location