CD180 antibody (APC)

Catalog No. ABIN2749113

Product Details anti-CD180 Antibody (APC)

Target: CD180 (CD180 Molecule (CD180))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD180 antibody is conjugated to APC

Application: Flow Cytometry (FACS)

Specificity: The mouse monoclonal antibody G28-8 recognizes an extracellular epitope of CD180, a 95-105 kDa TLR-like glycoprotein expressed on peripheral blood monocytes and dendritic cells, mantle zone B cells and marginal zone B cells, but very weakly on germinal center B cells.

Cross-Reactivity (Details): Human

Purification: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Human tonsillar B cells

Clone: G28-8

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Comment:

The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Restrictions: For Research Use only

Handling

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References for anti-CD180 antibody (APC) (ABIN2749113)

Olson, Graves, Shu, Ryan, Clark: "Caspase activity is required for stimulated B lymphocytes to enter the cell cycle." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 12, pp. 6065-72, (2003) (PubMed).

Clark, Shu, Lüscher, Draves, Banchereau, Ledbetter, Valentine: "Activation of human B cells. Comparison of the signal transduced by IL-4 to four different competence signals." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 143, Issue 12, pp. 3873-80, (1990) (PubMed).

Valentine, Clark, Shu, Norris, Ledbetter: "Antibody to a novel 95-kDa surface glycoprotein on human B cells induces calcium mobilization and B cell activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 140, Issue 12, pp. 4071-8, (1988) (PubMed).

Target Details for CD180

Target: CD180 (CD180 Molecule (CD180))

Alternative Name: CD180 (CD180 Products)

Background: CD180 Molecule,CD180, also known as RP105 (or Bgp95, LY64) is a type I membrane glycoprotein of Toll-like receptor (TLR) family. Its cytoplasmic tail is short and unlike the TLRs, it lacks the TIR domain. CD180 expression is dependent on the coexpression of its helper molecule, MD-1, and mirrors that of TLR4 on antigen-presenting cells. CD180 regulates recognition of LPS and signaling in B cells, via interacting directly with the TLR4 signaling complex, inhibiting its ability to bind microbial ligands. Ligation of CD180 by monoclonal antibodies leads to B cell activation, upregulation of CD80/CD86, and increase in cell size.,LY64, Ly78, RP105, Bgp95

Gene ID: 4064

UniProt: Q99467

Pathways: Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades