Sialoadhesin/CD169 antibody (PE)

Catalog No. ABIN2749112

Product Details anti-Sialoadhesin/CD169 Antibody (PE)

Target: Sialoadhesin/CD169 (SIGLEC1) (Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Sialoadhesin/CD169 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Specificity: The mouse monoclonal antibody 7-239 recognizes an extracellular epitope of CD169 (sialoadhesin, Siglec-1), a 210 kDa type I transmembrane glycoprotein expressed on macrophages and dendritic cells.

Cross-Reactivity (Details): Human

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: human rhinovirus 14-infected monocyte-derived dendritic cells

Clone: 7-239

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Comment:

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Restrictions: For Research Use only

Handling

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References for anti-Sialoadhesin/CD169 antibody (PE) (ABIN2749112)

Kawasaki, Vela, Nycholat, Rademacher, Khurana, van Rooijen, Crocker, Kronenberg, Paulson: "Targeted delivery of lipid antigen to macrophages via the CD169/sialoadhesin endocytic pathway induces robust invariant natural killer T cell activation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 19, pp. 7826-31, (2013) (PubMed).

Schrauf, Kirchberger, Majdic, Seyerl, Zlabinger, Stuhlmeier, Sachet, Seipelt, Stöckl: "The ssRNA genome of human rhinovirus induces a type I IFN response but fails to induce maturation in human monocyte-derived dendritic cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 7, pp. 4440-8, (2009) (PubMed).

Target Details for Sialoadhesin/CD169

Target: Sialoadhesin/CD169 (SIGLEC1) (Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1))

Alternative Name: CD169 / Siglec-1 (SIGLEC1 Products)

Synonyms: Cd169 antibody, Siglec-1 antibody, Sn antibody, CD169 antibody, SIGLEC-1 antibody, SN antibody, dJ1009E24.1 antibody, SA antibody, SIGLEC1 antibody, p210 antibody, pSn antibody, sialic acid binding Ig like lectin 1 antibody, sialic acid binding Ig-like lectin 1, sialoadhesin antibody, SIGLEC1 antibody, Siglec1 antibody

Background: Sialic acid binding Ig like lectin 1,CD169, also known as Siglec-1 or sialoadhesin, is a type I transmembrane glycoprotein of the sialic acid binding Ig-like lectin family. It binds to sialylated glycoproteins on various haematopoietic cells to mediate cell-cell interactions. CD169 is expressed on a subset of macrophages and dendritic cells. On CD14+ monocytes its expression can be induced by interferon alpha and gamma. High expression of CD169 is observed in the spleen, lymph nodes, bone marrow, and under inflammatory conditions rheumatoid arthritis and atherosclerosis, lower in the liver, lungs and gut. It has been shown to be involved in antigen presentation to invariant NKT cells, which play an important role in the innate arm of the immune system to modulate the subsequent acquired immune responses.,SIGLEC1, SN, sialoadhesin

Gene ID: 6614

UniProt: Q9BZZ2