ARHGEF4 antibody (AA 143-271) (PE)

Catalog No. ABIN2749098

Product Details anti-ARHGEF4 Antibody (PE)

Target: ARHGEF4 (rho Guanine Nucleotide Exchange Factor (GEF) 4 (ARHGEF4))

Binding Specificity: AA 143-271

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ARHGEF4 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Specificity: The mouse monoclonal antibody ARHGEF-08 recognizes human intracellular protein ARHGEF4 / ASEF1, approx. 80 kDa guanine nucleotide exchange factor specific for Rac1 and Cdc42. The epitope is located in the rough at the region of SH3 domain.

Cross-Reactivity (Details): Human

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Recombinant fragment of human ARHGEF4 (amino acids 143-271)

Clone: ARHGEF-08

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-5 μg/mL. Intracellular staining.

Comment:

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate was purified by size-exclusion chromatography.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Target Details for ARHGEF4

Target: ARHGEF4 (rho Guanine Nucleotide Exchange Factor (GEF) 4 (ARHGEF4))

Alternative Name: ARHGEF4 (ARHGEF4 Products)

Synonyms: ARHGEF4 antibody, ASEF antibody, ASEF1 antibody, GEF4 antibody, STM6 antibody, 9330140K16Rik antibody, Asef antibody, ENSMUSG00000070955 antibody, Rho guanine nucleotide exchange factor 4 antibody, uncharacterized LOC100470646 antibody, rho guanine nucleotide exchange factor 4 antibody, Rho guanine nucleotide exchange factor (GEF) 4 antibody, ARHGEF4 antibody, arhg4 antibody, LOC100470646 antibody, LOC100544917 antibody, Arhgef4 antibody

Background: Rho guanine nucleotide exchange factor 4,ARHGEF4 (Rho guanine nucleotide exchange factor 4), also known as ASEF 1 (adenomatous polyposis coli –, stimulated guanine nucleotide exchange factor 1) is an approximately 80 kDa cytoplasmic protein important for growth factor-mediated regulation of cell morphology and migration. Besides N-terminal adenomatous polyposis coli (APC)-binding region (ABR) it contains Dbl homology (DH), pleckstrin homology (PH) and SH3 domains. The SH3 domain inhibits GEF activity of ARHGEF4 by intramolecular interaction with the DH domain, whereas binding of APC stimulates the GEF activity. Activated ARHGEF4 stimulates the small GTPase Cdc42, which leads to decreased cell-cell adherence and enhanced cell migration.,ASEF1, GEF4, STM6

Gene ID: 50649

UniProt: Q9NR80

Pathways: Neurotrophin Signaling Pathway