PCLO antibody

Catalog No. ABIN2749094

Product Details anti-PCLO Antibody

Target: PCLO (Piccolo (Presynaptic Cytomatrix Protein) (PCLO))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PCLO antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Specificity: The mouse monoclonal antibody PCLO-01 recognizes PCLO (Piccolo), a more than 400 kDa multidomain protein expressed mainly in the presynaptic cytoplasmatic matrix of the neurons.

Cross-Reactivity (Details): Human

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Human recombinant PCLO protein

Clone: PCLO-01

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-5 μg/mL, intracellular staining.
Western blotting: A small splice variant of PCLO can be analyzed by Western blotting, however, the whole protein is too large (over 400 kDa) to be successfully processed by SDS PAAGE.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for PCLO

Target: PCLO (Piccolo (Presynaptic Cytomatrix Protein) (PCLO))

Alternative Name: PCLO (PCLO Products)

Background: Piccolo presynaptic cytomatrix protein,PCLO (piccolo, also known as aczonin) is a large (more than 400 kDa) multidomain protein of the presynaptic cytomatrix in neurons, that is present in all vertebrate synapses, but is absent from invertebrates. It contains zinc finger and coiled-coil sequences, as well as N-terminal glutamine-rich sequence, and C-terminal PDZ domain followed by two C2 domains (C2A and C2B). In vitro binding and transfection experiments suggested that PCLO binds to multiple proteins including profilin and L-type calcium channels. It is involved in neurotransmitter release and insulin secretion.,ACZ, PCH3, piccolo, aczonin

Gene ID: 27445

UniProt: Q9Y6V0

Pathways: Hormone Transport, Synaptic Vesicle Exocytosis