CD35 antibody

Catalog No. ABIN2749062

Product Details anti-CD35 Antibody

Target: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD35 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Specificity: The mouse monoclonal antibody E11 recognizes an extracellular epitope of CD35 (CR1), a type I transmembrane glycoprotein expressed on granulocytes, monocytes, B cells, folicular dendritic cells, erythrocytes, NK and T cell subsets, as well as e.g. on glomerulal podocytes.

Cross-Reactivity (Details): Human, Non-Human Primates

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Acute monocytic leukemia cells and normal blood monocytes

Clone: E11

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry (paraffin sections): Heat mediated antigen retrieval.
Immunohistochemistry (frozen sections): Acetone fixation.
Flow cytometry: Recommended dilution: 1-4 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CD35 antibody (ABIN2749062)

Hogg, Ross, Jones, Slusarenko, Walport, Lachmann: "Identification of an anti-monocyte monoclonal antibody that is specific for membrane complement receptor type one (CR1)." in: European journal of immunology, Vol. 14, Issue 3, pp. 236-43, (1984) (PubMed).

Target Details for CD35

Target: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Alternative Name: CD35 (CR1 Products)

Synonyms: C3BR antibody, C4BR antibody, CD35 antibody, KN antibody, C3DR antibody, CD21 antibody, Cr-1 antibody, Cr-2 antibody, Cr1 antibody, CR1-L antibody, CR2 antibody, CR1 antibody, complement C3b/C4b receptor 1 (Knops blood group) antibody, complement receptor 2 antibody, complement component (3d/Epstein Barr virus) receptor 2 antibody, complement receptor type 1 antibody, CR1 antibody, Cr2 antibody, CR2 antibody, LOC718120 antibody

Background: Complement C3b/C4b receptor 1 (Knops blood group),CD35 (complement receptor 1, CR1) is a monomeric multiple modular cell surface glycoprotein which serves as receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. It is expressed mainly on the surface of granulocytes, monocytes, erythrocytes, B cells and folicular dendritic cells. Besides its role in complement cascade, CD35 is involved in blocking BCR-induced proliferation and the differentiation of B cells to plasmablasts and their Ig production.,CR1, KN, C3BR, C4BR

Gene ID: 1378

UniProt: P17927

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Endopeptidase Activity