CD247 antibody (Tyr72)

Catalog No. ABIN2749060

Product Details anti-CD247 Antibody

Target: CD247 (CD247 Molecule (CD247))

Binding Specificity: Tyr72

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD247 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Specificity: The mouse monoclonal antibody EM-26 recognizes phosphorylated intracellular tyrosine 72 of CD3 zeta chain (CD247), which is a component of TCR/CD3 complex expressed on T cells.

Cross-Reactivity (Details): Human, Mouse

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Endotoxin Level: Low Endotoxin

Immunogen: A phospho specific peptide corresponding to the amino acids surrounding tyrosine 72 of mouse CD3 zeta linked to KLH

Clone: EM-26

Isotype: IgG2b

Application Details

Application Notes: Western blotting: Recommended dilution: 2 - 5 μg/mL, positive control: Jurkat cells lysate treated with pervanadate, splenocyte lysate of Balb/c or F1 mouse treated with pervanadate, non-reducing conditions recommended.
Flow cytometry: Intracellular staining, recommended dilution: 1-9 μg/mL, positive control: Jurkat cells treated with pervanadate, T-cells from lymph nodes of OT-1 mouse treated with pervanadate.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CD247 antibody (ABIN2749060)

Dopfer, Schöpf, Louis-Dit-Sully, Dengler, Höhne, Klescová, Prouza, Suchanek, Reth, Schamel: "Analysis of novel phospho-ITAM specific antibodies in a S2 reconstitution system for TCR-CD3 signalling." in: Immunology letters, Vol. 130, Issue 1-2, pp. 43-50, (2010) (PubMed).

Target Details for CD247

Target: CD247 (CD247 Molecule (CD247))

Alternative Name: CD3 zeta (CD247 Products)

Synonyms: CD3-ZETA antibody, CD3H antibody, CD3Q antibody, CD3Z antibody, T3Z antibody, TCRZ antibody, 4930549J05Rik antibody, A430104F18Rik antibody, AW552088 antibody, Cd3 antibody, Cd3-eta antibody, Cd3-zeta antibody, Cd3h antibody, Cd3z antibody, Cd3zeta antibody, T3z antibody, Tcrk antibody, Tcrz antibody, LOC100136514 antibody, LOC100136549 antibody, LOC100305023 antibody, CD247 antibody, TCRzeta antibody, CD247 molecule antibody, CD247 antigen antibody, CD3zeta-2 antibody, CD3zeta-1 antibody, Cd247 molecule antibody, CD247 antibody, Cd247 antibody, cd3z antibody, cd247 antibody

Background: CD247 Molecule,CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta (CD247). These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.,CD3 zeta chain, CD247, T3Z, TCRz, IMD25

Gene ID: 919

UniProt: P20963

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway