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CD28 antibody (FITC)

CD28 Reactivity: Rat FACS Host: Mouse Monoclonal JJ319 FITC
Catalog No. ABIN2749055
  • Target See all CD28 Antibodies
    CD28
    Reactivity
    • 169
    • 119
    • 54
    • 34
    • 22
    • 16
    • 12
    • 12
    • 7
    • 7
    • 5
    • 5
    • 1
    • 1
    • 1
    • 1
    Rat
    Host
    • 126
    • 77
    • 28
    • 16
    • 8
    • 3
    • 2
    Mouse
    Clonality
    • 182
    • 72
    • 3
    Monoclonal
    Conjugate
    • 89
    • 31
    • 27
    • 21
    • 14
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CD28 antibody is conjugated to FITC
    Application
    • 197
    • 90
    • 53
    • 47
    • 38
    • 32
    • 28
    • 26
    • 19
    • 17
    • 13
    • 7
    • 6
    • 6
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS)
    Specificity
    The mouse monoclonal antibody JJ319 reacts with an extracellular epitope of CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells.
    Cross-Reactivity (Details)
    Rat
    Purification
    Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
    Immunogen
    Mouse A20J B lymphoma cells transfected with rat CD28
    Clone
    JJ319
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product CD28 Primary Antibody
  • Application Notes
    Flow cytometry: Recommended dilution: 2 μg/mL.
    Comment

    The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.

    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
  • Guillonneau, Séveno, Dugast, Li, Renaudin, Haspot, Usal, Veziers, Anegon, Vanhove: "Anti-CD28 antibodies modify regulatory mechanisms and reinforce tolerance in CD40Ig-treated heart allograft recipients." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 12, pp. 8164-71, (2007) (PubMed).

    Kerstan, Armbruster, Leverkus, Hünig: "Cyclosporin A abolishes CD28-mediated resistance to CD95-induced apoptosis via superinduction of caspase-3." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 11, pp. 7689-97, (2006) (PubMed).

    van den Brandt, Wang, Reichardt: "Resistance of single-positive thymocytes to glucocorticoid-induced apoptosis is mediated by CD28 signaling." in: Molecular endocrinology (Baltimore, Md.), Vol. 18, Issue 3, pp. 687-95, (2004) (PubMed).

    Lühder, Huang, Dennehy, Guntermann, Müller, Winkler, Kerkau, Ikemizu, Davis, Hanke, Hünig: "Topological requirements and signaling properties of T cell-activating, anti-CD28 antibody superagonists." in: The Journal of experimental medicine, Vol. 197, Issue 8, pp. 955-66, (2003) (PubMed).

    Laskowski, Pratschke, Wilhelm, Dong, Beato, Taal, Gasser, Hancock, Sayegh, Tilney: "Anti-CD28 monoclonal antibody therapy prevents chronic rejection of renal allografts in rats." in: Journal of the American Society of Nephrology : JASN, Vol. 13, Issue 2, pp. 519-27, (2002) (PubMed).

    Haspot, Villemain, Laflamme, Coulon, Olive, Tiollier, Soulillou, Vanhove: "Differential effect of CD28 versus B7 blockade on direct pathway of allorecognition and self-restricted responses." in: Blood, Vol. 99, Issue 6, pp. 2228-34, (2002) (PubMed).

    Robinson, Clandinin, Field: "R3230AC rat mammary tumor and dietary long-chain (n-3) fatty acids change immune cell composition and function during mitogen activation." in: The Journal of nutrition, Vol. 131, Issue 7, pp. 2021-7, (2001) (PubMed).

    Dengler, Szabo, Sido, Nottmeyer, Zimmerman, Vahl, Hünig, Meuer: "Prolonged allograft survival but no tolerance induction by modulating CD28 antibody JJ319 after high-responder rat heart transplantation." in: Transplantation, Vol. 67, Issue 3, pp. 392-8, (1999) (PubMed).

    Tacke, Hanke, Hanke, Hünig: "CD28-mediated induction of proliferation in resting T cells in vitro and in vivo without engagement of the T cell receptor: evidence for functionally distinct forms of CD28." in: European journal of immunology, Vol. 27, Issue 1, pp. 239-47, (1997) (PubMed).

  • Target
    CD28
    Alternative Name
    CD28 (CD28 Products)
    Synonyms
    Tp44 antibody, CD28RNA antibody, cd28 antibody, CD28 molecule antibody, CD28 antigen antibody, Cd28 molecule antibody, CD28 molecule L homeolog antibody, CD28 antibody, Cd28 antibody, cd28.L antibody
    Background
    CD28 Molecule,CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.,Tp44
    Gene ID
    25660
    UniProt
    P31042
    Pathways
    TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
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