CD43 antibody

Catalog No. ABIN2749028

Product Details anti-CD43 Antibody

Target: CD43 (SPN) (Sialophorin (SPN))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD43 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Functional Studies (Func)

Specificity: The antibody MEM-59 recognizes a neuraminidase-sensitive extracellular epitope on CD43 (Leukosialin), a 95-135 kDa type I transmembrane glycoprotein (mucin-type) which is involved in lymphocyte activation. CD43 is expressed by platelets and at high levels on the surface of all leukocytes, it is negative on resting B lymphocytes and erythrocytes.

Cross-Reactivity (Details): Human

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Endotoxin Level: Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

Immunogen: Human T lymphocytes.

Clone: MEM-59

Isotype: IgG1

Application Details

Application Notes: Functional application: The antibody MEM-59 activates T lymphocytes and also induces apoptosis in hemopoietic progenitor cells.
Flow cytometry: Recommended dilution: 1-5 μg/mL.
Immunohistochemistry (paraffin sections): Positive tissue: spleen, thymus, tonsil, recommended dilution: 10 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CD43 antibody (ABIN2749028)

Schatzlmaier, Supper, Göschl, Zwirzitz, Eckerstorfer, Ellmeier, Huppa, Stockinger: "Rapid multiplex analysis of lipid raft components with single-cell resolution." in: Science signaling, Vol. 8, Issue 395, pp. rs11, (2015) (PubMed).

Símová, Klíma, Cermak, Sourková, Andera: "Arf and Rho GAP adapter protein ARAP1 participates in the mobilization of TRAIL-R1/DR4 to the plasma membrane." in: Apoptosis : an international journal on programmed cell death, Vol. 13, Issue 3, pp. 423-36, (2008) (PubMed).

Filatov, Krotov, Zgoda, Volkov: "Fluorescent immunoprecipitation analysis of cell surface proteins: a methodology compatible with mass-spectrometry." in: Journal of immunological methods, Vol. 319, Issue 1-2, pp. 21-33, (2007) (PubMed).

Cermák, Símová, Pintzas, Horejsí, Andera: "Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules." in: The Journal of biological chemistry, Vol. 277, Issue 10, pp. 7955-61, (2002) (PubMed).

Alvarado, Klassen, Cerny, Horejsí, Schmidt: "MEM-59 monoclonal antibody detects a CD43 epitope involved in lymphocyte activation." in: European journal of immunology, Vol. 25, Issue 4, pp. 1051-5, (1995) (PubMed).

Stefanová, Hilgert, Angelisová, Kristofová, Horejsí: "Characterization of a 95 kDa human leucocyte sialoglycoprotein: its identity with CD43, gpL115, leukosialin and sialophorin." in: Folia biologica, Vol. 34, Issue 4, pp. 255-65, (1989) (PubMed).

Target Details for CD43

Target: CD43 (SPN) (Sialophorin (SPN))

Alternative Name: CD43 (SPN Products)

Synonyms: CD43 antibody, GALGP antibody, GPL115 antibody, LSN antibody, A630014B01Rik antibody, Cd43 antibody, Galgp antibody, Ly-48 antibody, Ly48 antibody, Lsn antibody, Lsn1 antibody, gpL115 antibody, sialophorin antibody, SPN antibody, Spn antibody

Background: Sialophorin,CD43 (leukosialin, sialophorin) is a transmembrane mucin-like protein with high negative charge, expressed on the surface of most hematopoietic cells. CD43 contributes to a repulsive barrier that interferes with cellular adhesion, however, in certain cases also promotes leukocyte aggregation. By interaction with actin-binding proteins ezrin and moesin CD43 plays a regulatory role in remodeling T-cell morphology and regulates cell-cell interactions during lymphocyte traffic. CD43 signaling both enhances LFA-1 adhesiveness and counteracts LFA-1 induction via other receptors. Expression of CD43 causes induction of functionally active tumour suppressor p53 protein, but in case of p53 and ARF defficiency CD43 promotes tumour proliferation and viability. It appears to be an important modulator of leukocyte functions.,Leukosialin, Sialophorin, Galactoglycoprotein, GALGP, LSN, SPN, GALGP, GP5

Gene ID: 6693

UniProt: P16150

Pathways: Regulation of Leukocyte Mediated Immunity