CD16 antibody

Catalog No. ABIN2749016

Product Details anti-CD16 Antibody

Target: CD16

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD16 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies (Func), Western Blotting (WB)

Specificity: The antibody MEM-154 reacts with an extracellular epitope on CD16 antigen that is residing in proximity to FG loop (probably BC or C'E loop). CD16 is a low affinity receptor for aggregated IgG (FcgammaRIII antigen). The antibody MEM-154 reacts with CD16+ granulocytes, and it can be used for mapping CD16-158V/F polymorphism on NK cells, as it requires presence of V at amino acid 158.

Cross-Reactivity (Details): Human

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Endotoxin Level: Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

Immunogen: Human granulocytes

Clone: MEM-154

Isotype: IgG1

Application Details

Application Notes: Functional application: The antibody MEM-154 blocks binding of human IgG to FcγRIII.
Flow cytometry: Recommended dilution: 5-10 μg/mL, positive control: PBL (peripheral blood lymphocytes). The antibody MEM-154 does not react with CD16a present on NK cells in many subjects.
Western blotting: Non-reducing conditions.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CD16 antibody (ABIN2749016)

Gasdaska, Sherwood, Regan, Dickey: "An afucosylated anti-CD20 monoclonal antibody with greater antibody-dependent cellular cytotoxicity and B-cell depletion and lower complement-dependent cytotoxicity than rituximab." in: Molecular immunology, Vol. 50, Issue 3, pp. 134-41, (2012) (PubMed).

Koene, Kleijer, Algra, Roos, von dem Borne, de Haas: "Fc gammaRIIIa-158V/F polymorphism influences the binding of IgG by natural killer cell Fc gammaRIIIa, independently of the Fc gammaRIIIa-48L/R/H phenotype." in: Blood, Vol. 90, Issue 3, pp. 1109-14, (1997) (PubMed).

de Haas, Koene, Kleijer, de Vries, Simsek, van Tol, Roos, von dem Borne: "A triallelic Fc gamma receptor type IIIA polymorphism influences the binding of human IgG by NK cell Fc gamma RIIIa." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 156, Issue 8, pp. 2948-55, (1996) (PubMed).

Tamm, Schmidt: "The binding epitopes of human CD16 (Fc gamma RIII) monoclonal antibodies. Implications for ligand binding." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 4, pp. 1576-81, (1996) (PubMed).

Target Details for CD16

Target: CD16

Alternative Name: CD16 (CD16 Products)

Background: CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgammaRIII is expressed in two forms –, FcgammaRIII-A and -B. FcgammaRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgammaRIII-A is associated, moreover, with FcepsilonRI-beta subunit. Besides IgG, FcgammaRIII-A can be triggered also by oligomeric IgE. FcgammaRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.,FcgammaRIII, IGFR3, FCRIII

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process