CD13 antibody

Catalog No. ABIN2749014

Product Details anti-CD13 Antibody

Target: CD13 (ANPEP) (Alanyl (Membrane) Aminopeptidase (ANPEP))

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD13 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Functional Studies (Func)

Specificity: The antibody WM15 recognises an extracellular epitope of human CD13 cell surface glycoprotein, a 150 kDa molecule expressed on granulocytes, endothelial cells, epithelial cells and myeloid progenitors.

Cross-Reactivity (Details): Human, Non-Human Primates

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Endotoxin Level: Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

Immunogen: Human AML cells

Clone: WM15

Isotype: IgG1

Application Details

Application Notes: Functional application: The antibody WM15 inhibits infection of cells by human coronavirus and inhibits aminopeptidase N activity of the CD13 Molecule immunoprecipitates.
Flow cytometry: Recommended dilution: 1-4 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CD13 antibody (ABIN2749014)

McCormack, Muji?, Osdal, Bruserud, Gjertsen: "Multiplexed mAbs: a new strategy in preclinical time-domain imaging of acute myeloid leukemia." in: Blood, Vol. 121, Issue 7, pp. e34-42, (2013) (PubMed).

Favaloro, Browning, Facey: "CD13 (GP150; aminopeptidase-N): predominant functional activity in blood is localized to plasma and is not cell-surface associated." in: Experimental hematology, Vol. 21, Issue 13, pp. 1695-701, (1993) (PubMed).

Bradstock, Favaloro, Kabral, Kerr, Hughes, Berndt, Musgrove: "Human myeloid differentiation antigens identified by monoclonal antibodies: expression on leukemic cells." in: Pathology, Vol. 17, Issue 3, pp. 392-9, (1986) (PubMed).

Bradstock, Favaloro, Kabral, Kerr, Hughes, Musgrove: "Myeloid progenitor surface antigen identified by monoclonal antibody." in: British journal of haematology, Vol. 61, Issue 1, pp. 11-20, (1985) (PubMed).

Target Details for CD13

Target: CD13 (ANPEP) (Alanyl (Membrane) Aminopeptidase (ANPEP))

Alternative Name: CD13 (ANPEP Products)

Synonyms: APN antibody, CD13 antibody, GP150 antibody, LAP1 antibody, P150 antibody, PEPN antibody, A2AP antibody, AAP antibody, ALPHA-2-PI antibody, API antibody, PLI antibody, AAT1 antibody, ALT1 antibody, GPT1 antibody, Gpt1 antibody, Apn antibody, pepN antibody, SC8E4A.13 antibody, 1300007J06Rik antibody, 2310022B03Rik antibody, ALT antibody, Gpt-1 antibody, AP-M antibody, AP-N antibody, Cd13 antibody, Apm antibody, KZP antibody, Lap1 antibody, rAPN antibody, APDE antibody, p150 antibody, anpep antibody, fb64c05 antibody, wu:fb64c05 antibody, zgc:136771 antibody, alanyl aminopeptidase, membrane antibody, serpin family F member 2 antibody, glutamic--pyruvic transaminase antibody, aminopeptidase N antibody, aminopeptidase antibody, aminopeptidase n antibody, glutamic pyruvic transaminase, soluble antibody, alanyl (membrane) aminopeptidase antibody, membrane alanyl aminopeptidase antibody, alanyl (membrane) aminopeptidase b antibody, alanyl aminopeptidase, membrane L homeolog antibody, ANPEP antibody, SERPINF2 antibody, GPT antibody, Gpt antibody, Apn2 antibody, Apn1 antibody, SCO2643 antibody, pepN antibody, Shewana3_0897 antibody, Alvin_1024 antibody, Ndas_3413 antibody, Saut_1544 antibody, Fbal_1877 antibody, ML5_3424 antibody, Despr_1988 antibody, Mesop_5365 antibody, Anpep antibody, LOC101744250 antibody, anpepb antibody, anpep.L antibody

Background: Alanyl aminopeptidase, membrane,CD13 (aminopeptidase N, APN) is a 150 kDa type II transmembrane zinc-binding ectopeptidase expressed on various cell types. This metalloprotease preferentially catalyzes removal of neutral amino acids from small peptides, thus activating or inactivating bioactive peptides. CD13 has also role in extracellular matrix degradation, antigen processing and signal transduction, is important in inflammatory responses, regulates intercellular contact, cell motility and vascularization. CD13 is involved in protection of leukemic cells against apoptosis and its expression associated with poor prognosis of carcinomas.,Aminopeptidase N, APN, PEPN, ANPEP, gp150, LAP1

Gene ID: 290

UniProt: P15144

Pathways: Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones