Keratin 6 antibody (C-Term)

Catalog No. ABIN2742495

Product Details anti-Keratin 6 Antibody

Target: Keratin 6 (KRT6)

Binding Specificity: C-Term

Reactivity: Human, Chimpanzee, Gibbon, Horse, Orang-Utan

Host: Mouse

Clonality: Monoclonal

Conjugate: This Keratin 6 antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: Human KRT6 / CK6 / Cytokeratin 6

Purification: Protein G purified

Immunogen: A synthetic peptide of 11 amino acids (GSSTIKYTTTS) from the C-terminus of human Cytokeratin 6. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Baboon, Monkey, Horse (100%), Marmoset, Mouse, Rat, Ferret, Bat, Hamster, Elephant, Dog (91%), Sheep, Bovine (82%).

Type of Immunogen: Synthetic peptide

Clone: LHK6

Isotype: IgG2a kappa

Application Details

Application Notes: Approved: Flo (0.5 - 1 μg/10 x10⁶), IF (0.5 - 1 μg/mL), IHC, IHC-P (0.5 - 1 μg/mL)

Usage: This antibody recognizes a protein of 56  kDa, identified as cytokeratin 6 (CK6) or Keratin 6. In humans, multiple isoforms of Cytokeratin 6 (6A-6F), encoded by several highly homologous genes, have distinct tissue expression patterns, and Cytokeratin 6A is the dominant form in epithelial tissue. The gene encoding human Cytokeratin 6A maps to chromosome 12q13, and mutations in this gene are linked to several inheritable hair and skin pathologies. Keratins 6 and 16 are expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyper-proliferation-related keratins). Cytokeratin 6 is found in hair follicles, suprabasal cells of a variety of internal stratified epithelia, in epidermis, in both normal and hyper-proliferative situations. Epidermal injury results in activation of keratinocytes, which express Cytokeratin 6 and 16. Keratin 6 is strongly expressed in about 75 % of head and neck squamous cell carcinomas. Expression of Cytokeratin 6 is particularly associated with differentiation. ELISA: Order BSA-free antibody format for coating.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 mg/mL BSA, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2°C to 8°C.

Target Details for Keratin 6

Target: Keratin 6 (KRT6)

Alternative Name: KRT6 / CK6 / Cytokeratin 6 (KRT6 Products)

Synonyms: Krt2-6 antibody, Krt-2.6 antibody, keratin 6 antibody, Krt6 antibody