LGR5 antibody (AA 250-550)

Catalog No. ABIN2724692

Product Details anti-LGR5 Antibody

Target: LGR5 (Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5))

Binding Specificity: AA 250-550

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This LGR5 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Characteristics: Homo sapiens leucine-rich repeat containing G protein-coupled receptor 5 (LGR5)

Purification: Purified from mouse ascites fluids by affinity chromatography

Immunogen: Human recombinant protein fragment corresponding to amino acids 250-550 of human LGR5 (NP_003658) produced in HEK293T Cell.

Clone: 2A2

Isotype: IgG1

Application Details

Application Notes: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100, IP 2 μg/500 μL

Comment:

The concentration of the product may vary between diferrent lots.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5-1.0 mg/mL

Buffer: PBS (PH 7.3) containing 1 % BSA, 50 % glycerol and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-LGR5 antibody (ABIN2724692)

Patel, Sharon, Baranwal, Boothello, Desai, Patel: "Heparan sulfate hexasaccharide selectively inhibits cancer stem cells self-renewal by activating p38 MAP kinase." in: Oncotarget, Vol. 7, Issue 51, pp. 84608-84622, (2018) (PubMed).

Yang, Bavi, Wang, Roehrl: "Immuno-proteomic discovery of tumor tissue autoantigens identifies olfactomedin 4, CD11b, and integrin alpha-2 as markers of colorectal cancer with liver metastases." in: Journal of proteomics, Vol. 168, pp. 53-65, (2018) (PubMed).

Miyake, Li, Lee, Koike, Chen, Seo, Pierro: "Liver damage, proliferation, and progenitor cell markers in experimental necrotizing enterocolitis." in: Journal of pediatric surgery, Vol. 53, Issue 5, pp. 909-913, (2018) (PubMed).

Ma, Wang, Wang, Luo, Fan: "NANOGP8 is the key regulator of stemness, EMT, Wnt pathway, chemoresistance, and other malignant phenotypes in gastric cancer cells." in: PLoS ONE, Vol. 13, Issue 4, pp. e0192436, (2018) (PubMed).

Liu, Zheng, Ji, Liu, Hou, Li, Li: "Resveratrol reduces senescence-associated secretory phenotype by SIRT1/NF-κB pathway in gut of the annual fish Nothobranchius guentheri." in: Fish & shellfish immunology, Vol. 80, pp. 473-479, (2018) (PubMed).

Wang, Chen, Cao, Ma, Yin, Jia, Zang, Fan: "LGR5 Is a Gastric Cancer Stem Cell Marker Associated with Stemness and the EMT Signature Genes NANOG, NANOGP8, PRRX1, TWIST1, and BMI1." in: PLoS ONE, Vol. 11, Issue 12, pp. e0168904, (2017) (PubMed).

Osawa, Takahashi, Nishimura, Ohta, Haraguchi, Hata, Yamamoto, Mizushima, Takemasa, Doki, Mori: "Full-length LGR5-positive cells have chemoresistant characteristics in colorectal cancer." in: British journal of cancer, Vol. 114, Issue 11, pp. 1251-60, (2016) (PubMed).

Chen, Wei, Sun, Bhattacharya, Savage, Serda, Mackeyev, Curley, Bu, Wang, Chen, Cohen-Gould, Huang, Shen, Lipkin, Copeland, Jenkins, Shuler: "A recellularized human colon model identifies cancer driver genes." in: Nature biotechnology, Vol. 34, Issue 8, pp. 845-51, (2016) (PubMed).

Hsu, Liu, Tseng, Yang, Yeh, You, Hung, Chen, Chen: "CBB1003, a lysine-specific demethylase 1 inhibitor, suppresses colorectal cancer cells growth through down-regulation of leucine-rich repeat-containing G-protein-coupled receptor 5 expression." in: Journal of cancer research and clinical oncology, Vol. 141, Issue 1, pp. 11-21, (2015) (PubMed).

Shaikh, Zhou, Teo, Garg, Neogi, Figg, Yeo, Yu, Maguire, Zhao, Bennett, Azizan, Davenport, McKenzie, Brown: "LGR5 Activates Noncanonical Wnt Signaling and Inhibits Aldosterone Production in the Human Adrenal." in: The Journal of clinical endocrinology and metabolism, Vol. 100, Issue 6, pp. E836-44, (2015) (PubMed).

Jiang, Zhou, Chen, Schiff, Lee, Kong, Embree, Zhou, Mao: "Postnatal epithelium and mesenchyme stem/progenitor cells in bioengineered amelogenesis and dentinogenesis." in: Biomaterials, Vol. 35, Issue 7, pp. 2172-80, (2014) (PubMed).

Wang, Guo, Lui, Chu, Yoo, Chang, Yen: "Identification of a distinct small cell population from human bone marrow reveals its multipotency in vivo and in vitro." in: PLoS ONE, Vol. 9, Issue 1, pp. e85112, (2014) (PubMed).

Hsu, Liu, Tseng, Tan, Chen, Chen: "LGR5 regulates survival through mitochondria-mediated apoptosis and by targeting the Wnt/?-catenin signaling pathway in colorectal cancer cells." in: Cellular signalling, Vol. 26, Issue 11, pp. 2333-42, (2014) (PubMed).

Lühnsdorf, Epe, Khobta: "Excision of uracil from transcribed DNA negatively affects gene expression." in: The Journal of biological chemistry, Vol. 289, Issue 32, pp. 22008-18, (2014) (PubMed).

Wu, Xie, Gao, Wang, Guo, Tian, Li, Fan: "Lgr5 expression as stem cell marker in human gastric gland and its relatedness with other putative cancer stem cell markers." in: Gene, Vol. 525, Issue 1, pp. 18-25, (2013) (PubMed).

Pastò, Marchesi, Diamantini, Frasson, Curtarello, Lago, Pilotto, Parenti, Esposito, Agostini, Nitti, Amadori: "PKH26 staining defines distinct subsets of normal human colon epithelial cells at different maturation stages." in: PLoS ONE, Vol. 7, Issue 8, pp. e43379, (2012) (PubMed).

Target Details for LGR5

Target: LGR5 (Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5))

Alternative Name: LGR5 (LGR5 Products)

Synonyms: lgr5-a antibody, lgr5a antibody, FEX antibody, Gpr49 antibody, GPR49 antibody, GPR67 antibody, GRP49 antibody, HG38 antibody, leucine-rich repeat containing G protein-coupled receptor 5 L homeolog antibody, leucine rich repeat containing G protein coupled receptor 5 antibody, leucine rich repeat containing G protein-coupled receptor 5 antibody, lgr5.L antibody, Lgr5 antibody, LGR5 antibody

Background: LGR5 (leucine-rich repeat containing G protein-coupled receptor 5) An Orphan GPCR protein, contains large extracellular domain, Stem cell biomarker for small intestine, active in colon cancer, overexpressed in ovary and liver tumors.

Molecular Weight: 99.8 kDa

Gene ID: 8549

NCBI Accession: NM_003667

HGNC: 8549

Pathways: WNT Signaling