14-3-3 theta antibody
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- Target See all 14-3-3 theta (YWHAQ) Antibodies
- 14-3-3 theta (YWHAQ)
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This 14-3-3 theta antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificity
- This antibody detects endogenous levels of 14-3-3 θ protein. (region surrounding Gln67)
- Cross-Reactivity (Details)
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. - Purification
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
- Purity
- > 95 % pure by SDS-PAGE
- Top Product
- Discover our top product YWHAQ Primary Antibody
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- Application Notes
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ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1,0 mg/mL
- Buffer
- Phosphate Buffered Saline (PBS), pH ~7.2, 0.05 % Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- DO NOT FREEZE!
- Storage
- 4 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C.
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- Target
- 14-3-3 theta (YWHAQ)
- Alternative Name
- 14-3-3 Protein theta (YWHAQ Products)
- Synonyms
- 14-3-3 antibody, 14-3-3t antibody, 1c5 antibody, hs1 antibody, 1C5 antibody, HS1 antibody, 2700028P07Rik antibody, AA409740 antibody, AU021156 antibody, R74690 antibody, ywhaq antibody, ywhaq2 antibody, wu:fa91b01 antibody, wu:fb02e12 antibody, wu:fb36f06 antibody, wu:fk86h02 antibody, ywhaq1 antibody, ywhaz antibody, zgc:55499 antibody, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta antibody, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta antibody, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta S homeolog antibody, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide a antibody, 14-3-3 protein theta antibody, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide b antibody, YWHAQ antibody, ywhaq antibody, Ywhaq antibody, ywhaq.S antibody, ywhaqa antibody, ywhaqb antibody
- Background
- 14-3-3 proteins regulate many cellular processes relevant to cancer biology,notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 β, γ, ε, ζ, η, θ and σ. 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins, by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzymatic activity and facilitation of protein modification, and thus loss of expression contributes to a vast array of pathogenic cellular activitiesSynonyms: 14-3-3 protein T-cell, 14-3-3 protein tau, Protein HS1, YWHAQ
- Molecular Weight
- approx. 28 kDa
- Gene ID
- 10971
- NCBI Accession
- NP_006817
- UniProt
- P27348
- Pathways
- Apoptosis, Myometrial Relaxation and Contraction
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