TUBG1 antibody

Catalog No. ABIN271969

Product Details anti-TUBG1 Antibody

Target: TUBG1 (Tubulin, gamma 1 (TUBG1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TUBG1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: Tubulin gamma antibody detects endogenous levels of Tubulin gamma protein. (region surrounding Phe414)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: ELISA: 1/5000-1/10000. Western blot: 1/500-1/1000. Immunohistochemistry: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 15 mM Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for TUBG1

Target: TUBG1 (Tubulin, gamma 1 (TUBG1))

Alternative Name: TUBG1 / Tubulin gamma 1 (TUBG1 Products)

Background: TUB4, the gene for Saccharomyces cerevisiae gamma-Tubulin, encodes a 473 amino acid structural protein that localizes to the spindle pole body. gamma Tubulin, an essential protein for cell growth, organizes microtubule arrays in the nucleus and cytoplasm. gamma Tubulin-depleted cells fail to form functional spindles and arrest during nuclear division. gamma Tubulin associates with spindle body components Spc97 and Spc98 to form the gamma Tubulin complex. The budding yeast gamma Tubulin complex contains one molecule each of Spc97 and Spc98 and two molecules of gamma Tubulin. In the SPB, Spc110 binds Spc97 and Spc98 of the gamma Tubulin complex. 2D gel analysis indicates five isoforms of gamma Tubulin. The phosphorylation of gamma Tubulin at Tyr 445 plays a regulatory role in microtubule formation. The incidence rate for this phosphorylation event peaks during G1.Synonyms: GCP-1, Gamma-1-tubulin, Gamma-tubulin complex component 1, Tubulin gamma-1 chain

Molecular Weight: approx. 55 kDa

Gene ID: 7283

NCBI Accession: NP_001061

UniProt: P23258

Pathways: Microtubule Dynamics, M Phase