PAWR antibody

Catalog No. ABIN271791

Product Details anti-PAWR Antibody

Target: PAWR (PRKC, Apoptosis, WT1, Regulator (PAWR))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAWR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody detects endogenous levels of Prostate Apoptosis Response protein-4 protein. (region surrounding Asp313)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Purity: > 95 % pure by SDS-PAGE

Application Details

Application Notes: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: DO NOT FREEZE!

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for PAWR

Target: PAWR (PRKC, Apoptosis, WT1, Regulator (PAWR))

Alternative Name: PAR4 / PAWR (PAWR Products)

Synonyms: MGC81382 antibody, pawrl antibody, zgc:103513 antibody, PAWR antibody, PAR4 antibody, Par-4 antibody, Par4 antibody, 2310001G03Rik antibody, pro-apoptotic WT1 regulator antibody, PRKC, apoptosis, WT1, regulator L homeolog antibody, PRKC, apoptosis, WT1, regulator antibody, PAWR antibody, pawr.L antibody, pawr antibody, Pawr antibody

Background: Aberrant regulation of any of these cell processes can result in cancer. Cell death during embryogenesis, tissue atrophy and normal tissue turnover is called apoptosis and is characterized by cytoplasmic and nuclear condensation, nuclear disorganization and fragmentation of genomic DNA into 180-200 base pair oligomers. Five ionomycin-inducible complementary cDNAs, designated PAR1, 2, 3, 4 and 5, have been isolated from the prostate cancer cell line AT-3. Nucleotide sequencing identified PAR1 as the rat homolog of MKP-1, PAR2 as the injury-inducible gene HB-EGF, and PAR3 as the serum-induced gene Cyr61. PAR4 and PAR5 sequences were not found to correspond to any previously described proteins. PAR4 (prostate apoptosis response 4) is specifically expressed by cells entering apoptosis and is not induced during growth factor stimulation, oxidative stress, necrosis or growth arrest. The PAR4 gene encodes a protein with a putative nuclear localization signal and carboxy terminal leucine zipper.Synonyms: PRKC apoptosis WT1 regulator protein, Par-4, Prostate apoptosis response 4 protein

Molecular Weight: approx. 45 kDa

Gene ID: 5074

NCBI Accession: NP_002574

UniProt: Q96IZ0