ROR gamma T/RORC2/NR1F3 antibody

Catalog No. ABIN2689894

Product Details

Target: ROR gamma T/RORC2/NR1F3

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Intracellular Staining (ICS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: BD Pharmingen™

Characteristics: The Q31-378 monoclonal antibody recognizes RORgamma t (RORγt), an isoform of RORgamma (RORγ). RORγt is a DNA-binding transcription factor that belongs to the ROR/RZR orphan nuclear receptor family. RORγt is expressed exclusively by lymphoid cells including CD4+CD8+ thymocytes, peripheral CD4+ Th17 and CD8+ Tc17 cells, NKT cells and innate lymphoid cells such as lymphoid tissue inducer (LTi) cells. RORγt plays essential roles in thymopoiesis, T cell homeostasis, differentiation of effector T lymphocytes and the development of secondary lymphoid tissues including lymph nodes and Peyer's patches. Analyses of RORγt Expression by Western bloting and Immunohistochemistry. Left Panel: Western blot analyses of RORγt expression by mouse splenocytes and thymocytes. Cell lysates from untreated mouse splenocytes (Spl, Lanes 1,2) and thymocytes (Thy, Lanes 3,4) (20 μg total cellular protein/lane) were electrophoresed (SDS-PAGE) and transferred to membranes. They were then probed with Purified Anti-Actin antibody (Cat. No. 612656/612657, Lanes 1,3 at 1 μg of antibody/mL) or Purified Mouse Anti-Mouse RORγt antibody (

BD Pharmingen™ Purified Mouse anti-Mouse RORγt - Purified - Clone Q31-378 - Isotype Mouse IgG2a, κ - Reactivity Ms, Hu - 50 μg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse RORgammat Recombinant Protein

Clone: Q31

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References

Unutmaz: "RORC2: the master of human Th17 cell programming." in: European journal of immunology, Vol. 39, Issue 6, pp. 1452-5, (2009) (PubMed).

Hamada, Garcia-Hernandez, Reome, Misra, Strutt, McKinstry, Cooper, Swain, Dutton: "Tc17, a unique subset of CD8 T cells that can protect against lethal influenza challenge." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 6, pp. 3469-81, (2009) (PubMed).

Bechtel, Rosenfelder, Duda, Schmidt, Ernst, Wellenreuther, Mehrle, Schuster, Bahr, Blöcker, Heubner, Hoerlein, Michel, Wedler, Köhrer, Ottenwälder, Poustka, Wiemann, Schupp: "The full-ORF clone resource of the German cDNA Consortium." in: BMC genomics, Vol. 8, pp. 399, (2008) (PubMed).

Ivanov, McKenzie, Zhou, Tadokoro, Lepelley, Lafaille, Cua, Littman: "The orphan nuclear receptor RORgammat directs the differentiation program of proinflammatory IL-17+ T helper cells." in: Cell, Vol. 126, Issue 6, pp. 1121-33, (2006) (PubMed).

Eberl, Littman: "The role of the nuclear hormone receptor RORgammat in the development of lymph nodes and Peyer's patches." in: Immunological reviews, Vol. 195, Issue 9, pp. 81-90, (2003) (PubMed).

Eberl, Marmon, Sunshine, Rennert, Choi, Littman: "An essential function for the nuclear receptor RORgamma(t) in the generation of fetal lymphoid tissue inducer cells." in: Nature immunology, Vol. 5, Issue 1, pp. 64-73, (2003) (PubMed).

He, Deftos, Ojala, Bevan: "RORgamma t, a novel isoform of an orphan receptor, negatively regulates Fas ligand expression and IL-2 production in T cells." in: Immunity, Vol. 9, Issue 6, pp. 797-806, (1999) (PubMed).

Medvedev, Yan, Hirose, Giguère, Jetten: "Cloning of a cDNA encoding the murine orphan receptor RZR/ROR gamma and characterization of its response element." in: Gene, Vol. 181, Issue 1-2, pp. 199-206, (1997) (PubMed).

Hirose, Smith, Jetten: "ROR gamma: the third member of ROR/RZR orphan receptor subfamily that is highly expressed in skeletal muscle." in: Biochemical and biophysical research communications, Vol. 205, Issue 3, pp. 1976-83, (1995) (PubMed).

Target Details

Target: ROR gamma T/RORC2/NR1F3

Alternative Name: RORgammat

Background: Synonyms: RORγT, RORgt, RORgamma t, RORgammaT, Rorc2, Rorg, TOR, Thor, Nr1f3