R-Phycoerythrin antibody (Biotin)

Catalog No. ABIN2689889

Product Details anti-R-Phycoerythrin Antibody (Biotin)

Target: R-Phycoerythrin (Phycoerythrin, R-)

Host: Mouse

Clonality: Monoclonal

Conjugate: This R-Phycoerythrin antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Brand: BD Pharmingen™

Characteristics: The E31-1459 antibody reacts with R-Phycoerythrin (PE), a commonly used fluorochrome for flow cytometry, alone or as a tandem conjugate with other fluorochromes (eg, PE-Cy™7). The binding of the E31-1459 antibody to PE does not quench the fluorescence of the PE molecule. The use of biotinylated E31-1459 in a three-step staining procedure with Streptavidin-PE and a PE-conjugated primary antibody can specifically amplify the PE fluorescence. Amplification of PE-conjugated anti-mouse CD19 staining of BALB/c mouse splenocytes. Mouse splenocytes were incubated with PE-conjugated anti-mouse CD19 mAb 1D3 (Cat. No. 557399/553786) alone or followed by biotinylated mAb E31- 1459 and PE-conjugated Streptavidin (Cat. No. 554061, right panel). The overlayed histograms correspond to stained splenocytes (shaded histogram), unstained splenocytes (left panel, unshaded histogram), or splenocytes stained with biotinylated mAb E31-1459 and PE-conjugated Streptavidin (right panel, unshaded histogram). The signal-to-noise ratio of median fluorescence intensity (MFI) of the CD19+ population to the CD19- population in both panels (shaded histograms) is 87 for Panel A and 172 for right panel, demonstrating an approximate two-fold specific amplification of staining. Data shown is for gated lymphocytes based on light scatter. Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.

BD Pharmingen™ Biotin Mouse Anti-R-Phycoerythrin - Biotin - Clone E31-1459 - Isotype Mouse IgG1, κ - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: R-phycoerythrin

Clone: E31

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Target Details for R-Phycoerythrin

Target: R-Phycoerythrin (Phycoerythrin, R-)

Alternative Name: R-Phycoerythrin (R-Phycoerythrin Products)

Target Type: Chemical