Qa-2 antibody (Biotin)

Catalog No. ABIN2689888

Product Details anti-Qa-2 Antibody (Biotin)

Target: Qa-2

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Qa-2 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Brand: BD Pharmingen™

Characteristics: The 1-1-2 antibody reacts with the Qa-2 MHC class Ib antigen, which is expressed at higher levels on mature T lymphocytes than on B cells. Its expression is upregulated in the late stages of T-cell maturation. Qa-2 is a 40 kDa GPI-linked cell surface molecule encoded by the Q5, Q6, Q7, and/or Q9 genes of the H-2 complex. A 39 kDa soluble form results from alternative splicing of the transcript. Although Qa-2 is nonpolymorphic, its level of expression on both T and B lymphocytes varies among inbred mouse strains. High expressors include strains carrying the H-2[b] haplotype low expressors include H-2[d] strains, and H-2[k] strains are Qa-2-negative.

BD Pharmingen™ Biotin Mouse Anti-Mouse Qa-2 - Biotin - Clone 1-1-2 - Isotype Mouse IgG2a, κ - Reactivity Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: C3H.SW mouse skin graft and splenocytes

Clone: 1-1

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-Qa-2 antibody (Biotin) (ABIN2689888)

Gabor, Godfrey, Scollay: "Recent thymic emigrants are distinct from most medullary thymocytes." in: European journal of immunology, Vol. 27, Issue 8, pp. 2010-5, (1997) (PubMed).

Shawar, Vyas, Rodgers, Rich: "Antigen presentation by major histocompatibility complex class I-B molecules." in: Annual review of immunology, Vol. 12, pp. 839-80, (1994) (PubMed).

Sharrow, Arn, Stroynowski, Hood, Sachs: "Epitope clusters of Qa-2 antigens defined by a panel of new monoclonal antibodies." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 10, pp. 3495-502, (1989) (PubMed).

Target Details for Qa-2

Target: Qa-2

Abstract: Qa-2 Products