Western Blotting (WB), Immunofluorescence (IF), BioImaging (BI), Intracellular Staining (ICS)
Brand
BD Pharmingen™
Characteristics
The R11-560 monoclonal antibody specifically binds to human and mouse homeobox protein Nkx6.1. The transcription factor Nkx6.1 plays a role in pancreatic islet beta cell development through the regulation of HNF1a and insulin genes. Nkx6.1 is considered to be a highly specific transcription factor marking pancreatic beta islet cells, where it is expressed in both developing and mature cells. In addition, Nkx6.1 functions as a transcriptional repressor induced by the Sonic Hedgehog (Shh) signaling pathway in fate determination of several neuronal cell types. During antibody development, the purified R11-560 monoclonal antibody was found to detect Nkx6.1 by western blot and indirect immunofluorescent staining followed by flow cytometric analysis or imaging analysis. Western Blot analysis of Nkx6.1 in mouse pancreatic tumor (insulinoma) cell lysate. Lysate from Beta-T-C6 cells (ATCC, CRL-11506™) was probed with Purified Mouse Anti-Nkx6.1 monoclonal antibody at titrations of 0.5 (lane 1), 0.25 (lane 2), and 0.125 μg/mL (lane 3). Nkx6.1 is identified as a doublet of approximately 44 kDa. Immunoflourescent staining of Nkx6.1 in mouse pancreatic tumor (insulinoma) cells. Beta-T-C6 cells (ATCC, CRL-11506™) were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655), permeabilized with BD Phosflow™ Perm III Buffer (Cat. No. 558050, and stained with Purified Mouse Anti-Nkx6.1 monoclonal antibody (pseudo-colored green) at 1.2 μg/mL. The second-step reagent was Alexa Fluor® 488 goat anti-mouse Ig (Life Technologies), and counter-staining was with DAPI (pseudo-colored blue). The images were captured on a BD Pathway™ 435 Cell Analyzer and merged using BD AttoVision™ Software.
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C
Storage Comment
Store undiluted at 4°C.
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