Sca-1/Ly-6A/E antibody

Catalog No. ABIN2689819

Product Details anti-Sca-1/Ly-6A/E Antibody

Target: Sca-1/Ly-6A/E (Ly6a) (Lymphocyte Antigen 6 Complex, Locus A (Ly6a))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Sca-1/Ly-6A/E antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Cytotoxicity Test (CyTox)

Brand: BD Pharmingen™

Characteristics: The E13-161.7 antibody reacts with Ly-6A.2 and Ly-6E.1, which are allelic members of the Ly-6 multigene family. Sca1 (Ly-6A/E), a phosphatidylinositol-anchored protein of about 18 kDa, is expressed on the multipotent hematopoietic stem cell (HSC) in mice with both Ly-6 haplotypes. Sca-1+ HSC are found in the adult bone marrow and fetal liver, but not in the early embryo yolk sac or intraembryonic hematopoietic sites, and can be mobilized to the peripheral blood and spleen in the adult. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, C57L, C58, DBA/2, PL, SJL, SWR, 129), Ly-6A/E is also expressed on distinct subpopulations of bone marrow and peripheral B lymphocytes, myeloid cells, and thymic and peripheral T lymphocytes, on the earliest intrathymic T-cell precursor population, and in several non-hematopoietic tissues. Strains with the Ly-6.1 haplotype (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Ly-6A/E+ resting peripheral lymphocytes, whereas activation of T cells from mice of both Ly-6 haplotypes leads to strong expression of the Sca-1 antigen. Studies with the D7 antibody (Cat. No. 557403) have demonstrated that Ly-6A/E may be involved in the regulation of B and T lymphocyte responses, and it appears to be required for T-cell receptor-mediated T-cell activation. Purified E13-161.7 mAb can block binding of FITC-conjugated D7 antibody (anti-Ly-6A/E, Cat. No. 557405) to mouse splenocytes, but purified mAb D7 (Cat. No. 557403) is unable to block binding of FITC-conjugated E13-161.7 antibody (Cat. No. 553335). Anti-Ly-6A/E (Sca-1) mAb may be used in combination with the Mouse Lineage Panel (Cat. No. 559971) to identify HSC. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.

BD Pharmingen™ Purified Rat Anti-Mouse Ly-6A/E - Purified - Clone E13-161.7 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: BALB/c mouse-derived "pre-T" cell hybridoma

Clone: E13

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-Sca-1/Ly-6A/E antibody (ABIN2689819)

Kawamoto, Ohmura, Katsura: "Direct evidence for the commitment of hematopoietic stem cells to T, B and myeloid lineages in murine fetal liver." in: International immunology, Vol. 9, Issue 7, pp. 1011-9, (1997) (PubMed).

Morrison, Wandycz, Hemmati, Wright, Weissman: "Identification of a lineage of multipotent hematopoietic progenitors." in: Development (Cambridge, England), Vol. 124, Issue 10, pp. 1929-39, (1997) (PubMed).

Osawa, Nakamura, Nishi, Takahasi, Tokuomoto, Inoue, Nakauchi: "In vivo self-renewal of c-Kit+ Sca-1+ Lin(low/-) hemopoietic stem cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 156, Issue 9, pp. 3207-14, (1996) (PubMed).

Yamamoto, Yasumizu, Amou, Watanabe, Nishio, Toki, Fukuhara, Ikehara: "Characterization of peripheral blood stem cells in mice." in: Blood, Vol. 88, Issue 2, pp. 445-54, (1996) (PubMed).

Auerbach, Huang, Lu: "Hematopoietic stem cells in the mouse embryonic yolk sac." in: Stem cells (Dayton, Ohio), Vol. 14, Issue 3, pp. 269-80, (1996) (PubMed).

Morrison, Hemmati, Wandycz, Weissman: "The purification and characterization of fetal liver hematopoietic stem cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 22, pp. 10302-6, (1995) (PubMed).

Morrison, Weissman: "The long-term repopulating subset of hematopoietic stem cells is deterministic and isolatable by phenotype." in: Immunity, Vol. 1, Issue 8, pp. 661-73, (1995) (PubMed).

Spangrude, Brooks: "Mouse strain variability in the expression of the hematopoietic stem cell antigen Ly-6A/E by bone marrow cells." in: Blood, Vol. 82, Issue 11, pp. 3327-32, (1994) (PubMed).

Ivanov, Fleming, Malek: "Regulation of nuclear factor-kappa B and activator protein-1 activities after stimulation of T cells via glycosylphosphatidylinositol-anchored Ly-6A/E." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 6, pp. 2394-406, (1994) (PubMed).

Jurecic, Van, Belmont: "Enrichment and functional characterization of Sca-1+WGA+, Lin-WGA+, Lin-Sca-1+, and Lin-Sca-1+WGA+ bone marrow cells from mice with an Ly-6a haplotype." in: Blood, Vol. 82, Issue 9, pp. 2673-83, (1993) (PubMed).

Wu, Antica, Johnson, Scollay, Shortman: "Developmental potential of the earliest precursor cells from the adult mouse thymus." in: The Journal of experimental medicine, Vol. 174, Issue 6, pp. 1617-27, (1992) (PubMed).

Flood, Dougherty, Ron: "Inhibition of Ly-6A antigen expression prevents T cell activation." in: The Journal of experimental medicine, Vol. 172, Issue 1, pp. 115-20, (1990) (PubMed).

Codias, Malek: "Regulation of B lymphocyte responses to IL-4 and IFN-gamma by activation through Ly-6A/E molecules." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 6, pp. 2197-204, (1990) (PubMed).

Codias, Rutter, Fleming, Malek: "Down-regulation of IL-2 production by activation of T cells through Ly-6A/E." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 145, Issue 5, pp. 1407-14, (1990) (PubMed).

Spangrude, Klein, Heimfeld, Aihara, Weissman: "Two monoclonal antibodies identify thymic-repopulating cells in mouse bone marrow." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 2, pp. 425-30, (1989) (PubMed).

van de Rijn, Heimfeld, Spangrude, Weissman: "Mouse hematopoietic stem-cell antigen Sca-1 is a member of the Ly-6 antigen family." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, Issue 12, pp. 4634-8, (1989) (PubMed).

Spangrude, Aihara, Weissman, Klein: "The stem cell antigens Sca-1 and Sca-2 subdivide thymic and peripheral T lymphocytes into unique subsets." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 141, Issue 11, pp. 3697-707, (1988) (PubMed).

Spangrude, Heimfeld, Weissman: "Purification and characterization of mouse hematopoietic stem cells." in: Science (New York, N.Y.), Vol. 241, Issue 4861, pp. 58-62, (1988) (PubMed).

Aihara, Bühring, Aihara, Klein: "An attempt to produce "pre-T" cell hybridomas and to identify their antigens." in: European journal of immunology, Vol. 16, Issue 11, pp. 1391-9, (1987) (PubMed).

Malek, Ortega, Chan, Kroczek, Shevach: "Role of Ly-6 in lymphocyte activation. II. Induction of T cell activation by monoclonal anti-Ly-6 antibodies." in: The Journal of experimental medicine, Vol. 164, Issue 3, pp. 709-22, (1986) (PubMed).

Target Details for Sca-1/Ly-6A/E

Target: Sca-1/Ly-6A/E (Ly6a) (Lymphocyte Antigen 6 Complex, Locus A (Ly6a))

Alternative Name: Ly-6A/E (Ly6a Products)

Synonyms: Ly-6A.2 antibody, Ly-6A/E antibody, Ly-6E.1 antibody, Sca-1 antibody, Sca1 antibody, TAP antibody, lymphocyte antigen 6 complex, locus A antibody, Ly6a antibody

Background: Synonyms: Sca-1

Pathways: Sensory Perception of Sound, Activated T Cell Proliferation