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I-AD antibody (Biotin)

Reactivity: Mouse FACS, IHC (fro), IHC (p) Host: Mouse Monoclonal AMS Biotin
Catalog No. ABIN2689570
  • Target
    I-AD
    Reactivity
    Mouse
    Host
    • 1
    Mouse
    Clonality
    • 1
    Monoclonal
    Conjugate
    • 1
    Biotin
    Application
    Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Brand
    BD Pharmingen™
    Characteristics
    The AMS-32.1 antibody reacts with the I-A[d] MHC class II alloantigen. It cross-reacts with cells from mice of the H-2[f], H-2[g7], H-2[i], and H-2[v] haplotypes. Reactivity with other haplotypes (e.g., k, p, q, r, s, u) has not been observed. Immunohistochemistry of I-A[d] positive cells. Frozen sections of mouse thymus were reacted with the anti-I-A[d] antibody. Cells expressing the MHC class II molecule I-A[d] can be identified by the intense brown labeling of their cell membranes. Magnification 20x.

    BD Pharmingen™ Biotin Mouse Anti-Mouse I-A[d] - Biotin - Clone AMS-32.1 - Isotype Mouse IgG2b, κ - Reactivity Ms - 1 mL
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    BALB/c mouse splenocytes
    Clone
    AMS
    Isotype
    IgG2b kappa
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    62.5 μg/mL
    Buffer
    Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
    Storage
    4 °C
    Storage Comment
    Store undiluted at 4°C.
  • Target
    I-AD
    Alternative Name
    I-A[d]
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