HLA-DM antibody

Catalog No. ABIN2689553

Product Details anti-HLA-DM Antibody

Target: HLA-DM (MHC Class II HLA-DM (HLA-DM))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HLA-DM antibody is un-conjugated

Application: Intracellular Staining (ICS)

Brand: BD Pharmingen™

Characteristics: Reacts with human leukocyte antigen-DM (HLA-DM), a non-classical MHC class II molecule expressed in the cytoplasm of antigen presenting cells (APC). HLA-DM is composed of alpha and beta subunits which form a similar structure as the classical class II molecules. HLA-DM catalyzes the dissociation of CLIP from MHC class II-CLIP complexes in vitro and facilitates the binding of antigenic peptides. It also prevents self-antigens from becoming stably complexed with class II molecules and being presented to T cells. Profile of intracellular staining of peripheral blood lymphocytes analyzed on a FACScan . Profile of intracellular staining of peripheral blood monocytes analyzed on a FACScan .

BD Pharmingen™ Purified Mouse Anti-Human HLA-DM - Purified - Clone MaP.DM1 - Isotype Mouse IgG1, κ - Reactivity Hu - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Clone: MaP-DM1

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-HLA-DM antibody (ABIN2689553)

Denzin, Hammond, Cresswell: "HLA-DM interactions with intermediates in HLA-DR maturation and a role for HLA-DM in stabilizing empty HLA-DR molecules." in: The Journal of experimental medicine, Vol. 184, Issue 6, pp. 2153-65, (1997) (PubMed).

Kropshofer, Hämmerling, Vogt: "How HLA-DM edits the MHC class II peptide repertoire: survival of the fittest?" in: Immunology today, Vol. 18, Issue 2, pp. 77-82, (1997) (PubMed).

Denzin, Cresswell: "HLA-DM induces CLIP dissociation from MHC class II alpha beta dimers and facilitates peptide loading." in: Cell, Vol. 82, Issue 1, pp. 155-65, (1995) (PubMed).

Denzin, Robbins, Carboy-Newcomb, Cresswell: "Assembly and intracellular transport of HLA-DM and correction of the class II antigen-processing defect in T2 cells." in: Immunity, Vol. 1, Issue 7, pp. 595-606, (1995) (PubMed).

Riberdy, Avva, Geuze, Cresswell: "Transport and intracellular distribution of MHC class II molecules and associated invariant chain in normal and antigen-processing mutant cell lines." in: The Journal of cell biology, Vol. 125, Issue 6, pp. 1225-37, (1994) (PubMed).

Riberdy, Newcomb, Surman, Barbosa, Cresswell: "HLA-DR molecules from an antigen-processing mutant cell line are associated with invariant chain peptides." in: Nature, Vol. 360, Issue 6403, pp. 474-7, (1992) (PubMed).

Target Details for HLA-DM

Target: HLA-DM (MHC Class II HLA-DM (HLA-DM))

Alternative Name: HLA-DM